Research: Myeloid Derived-Suppressor Cells as A Potential Target of Immunotherapy in Notch Dependent T-Cell Acute Lymphoblastic Leukemia
WORK ADDRESS: Viale Regina Elena 291, 00161 – Roma
Tel +39 0649255673
SUPERVISOR: Antonio Francesco Campese
LANGUAGES
Persian - Mother Tongue
English - Fluent
Italian - Fluent
PREVIOUS RESEARCH EXPERIENCE
Università la Sapienza, Roma, Italy | ONGOING
1. Doctoral student of Molecular Medicine holder of doctoral fellowship in pathology molecular laboratory, at the Department of Molecular Medicine
Role of the NOTCH receptor: 1) in the development of acute T-cell
leukemia, 2)in the generation and function of myeloid-derived suppressor cells (MDSCs), and their crosstalk with NK cells, and possibly with
other TME cell subgroups, such as Treg and follicular-helper-T-'like' cells 3) in modulating the expression of the PD1/PD-L1 axis, with a potential role of these molecules in T-ALL immunotherapy.
2. Weill Cornell Medicine Medical College, New York USA | 2023 Fellowship as a mobility grant holder, in the prestigious Laboratory directed by Prof. Roberta Zappasodi, at the Division of Hematology and Medical Oncology
Investigation of the impact of tumor glycolysis and inhibition of immunosuppressive pathways, including those mediated by T-follicular- helper "like" cells and Myeloid-Derived Suppressor Cells (MDSCs) in the response to immunotherapy
3. Università la Sapienza, Roma, Italy | 2019 - 2020
Assistant Researcher
At the DIP. Molecular Medicine in the group of Prof. Antonio Francesco Campese, PhD.
4. Università la Sapienza, Roma, Italy | 2018 - 2019
Clinical Research Internship
at the Department of Translational and Precision Medicine in the group of Prof. Stefano Ginanni Corradini MD, PhD
5. Salamat Aval Teheran Medical Center & Akhtar Hospital, Iran | 2015- 2016
Clinical Assistant and clinical activity
at the Diagnostic Laboratory of Hematology and Biochemistry
EDUCATION HISTORY
1. Università la Sapienza, Roma, Italy | 2022
Preclinical Experimentation and Animal Welfare.
2. Università la Sapienza, Roma, Italy | 2016 - 2019
Master’s Degree in Medical Biotechnology
Thesis title "Genetic Predisposition and Potential Involvement of Lysosomal Acid Lipase in Metabolic Dysregulation in Individuals with Liver Disease of Various Etiologies"
3. Azad University, North TehranBranch, Iran | 2011 - 2015
Four-year Bachelor of Science in Cellular Molecular Biology in Microbiology
SCHOLARSHIPS
1. Università la Sapienza, Roma, Italy | 2021
Participation in “THE INFLUENCE OF TUMOR MICROENVIRONMENT
IN THE PROGRESSION OF NOTCH-DEPENDENT T-CELL ACUTE LYMPHOB LEUKEMIA: EXPLORING THE ROLE OF THE PD-
1/PD-L1 AXIS”
2. Weill Cornell Medicine Medical College, New York, USA | 2022 - 2023 Winner of the research project “'Exploring the possible role of PD1
immune-checkpoint in the treatment of Notch-dependent 'T-cell acute lymphoblastic leukemia”
3. Università la Sapienza, Roma, Italy | 2023
Participation in “Inhibitors of anti-apoptotic Bcl-2 proteins in the treatment of Notch-dependent T-cell Acute Lymphoblastic Leukemia
SCIENTIFIC PUBLICATIONS
Grazioli P, Orlando A, Giordano N, Noce C, Peruzzi G, Abdollahzadeh B, Screpanti I, Campese AF. Notch-Signaling Deregulation Induces Myeloid-Derived Suppressor Cells in T-Cell Acute Lymphoblastic Leukemia. Front Immunol. 2022 Apr 4;13:809261. doi: 10.3389/fimmu.2022.809261. PMID: 35444651; PMCID: PMC9013886
CONFERENCES
1. "MDSC/NK CELLS CROSS-TALK IN NOTCH-DEPENDENT T-CELL ACUTE LYMPHOBLASTIC LEUKEMIA: THE POSSIBLE ROLE OF THE PD-1/PD-L1 PATHWAY," at the Gordon Research Conference on Notch Signaling in Development, Regeneration and Disease held 07/17/2022 - 07/22/2022 at Bates College in Lewiston, Maine, United States
2. "EXPLORING THE ROLE OF NF-kB1/p50 IN NOTCH-DEPENDENT INDUCTION OF MDSCs IN T-CELL ACUTE LYMPHOBLASTIC LEUKEMIA MICROENVIRONMENT" at "62nd Annual Meeting of the Italian Cancer Society , Venice, 16-18 November 2022"
3. "MYELOID-DERIVED SUPPRESSOR CELLS AS A POTENTIAL TARGET OF IMMUNOTHERAPY IN NOTCH-DEPENDENT T-CELL ACUTE LYMPHOBLASTIC LEUKEMIA" at "the Notch Meeting XII” which took place in Athens, Greece from October 1st to October 5th, 2023.
RELEVANT SKILLS
Staining and analysis of cell samples by Flow-cytometry (BD Symphony) and FlowJo
Cell sample treatments and analysis using the Celigo Image Cytometer. Experience in vitro and in vivo suppression assays
Separation of cell populations from peripheral blood and murine organs, by both magnetic bead’s protocols and FACS-assisted cell sorting experiments
Isolation of blood cell populations from peripheral venous blood through differential centrifugation and density gradient centrifugation techniques
Perform with tissues from genetically modified mouse models Pharmacological treatment
Statistical skills working with SPSS, PRISM
Knowledge and experience with RNA/DNA and protein extraction PCR,qPCR assays and analysis
Expertise in cell culture and basic cellular and molecular biology techniques
ELISA assays
Demonstrated expertise in performing Chromatin Immunoprecipitation (ChIP) methodologies, proficiently investigating chromatin modifications and protein-DNA interactions
Experience in functional genomics (siRNA)
Cell Transfusion Technique (NEON SYSTEM TRANSFECTION)
Western blot technique
Experience in handling patient samples
Acquisition of blood sampling from peripheral venous blood Bacteriology techniques (Culture of aerobic, microaerophilic, and
anaerobic microorganisms)
COMUNICATION SKILLS
Strong ability to integrate into multicultural environments with a pronounced team spirit
Excellent communication and interpersonal skills
Ability to search, interpret and communicate scientific data Determined and outcome-focused professional, driven by a proven history of achieving success
Comprehensive familiarity and demonstrated proficiency in navigating immunotherapy microenvironments
Strong knowledge and understanding in the oncology therapy area, particularly within solid tumors and hematological malignancies Ability to comprehensively learn about new subject areas and environments
Good organizational and time management skills
An ability to take initiative and work both independently and in a team environment
Ability to actively listen and process information to identify opportunities Excellent written and spoken communication and presentation skills, with a demonstrated ability to develop and maintain strong collaborative relationships
Team player; ability to share good practices and knowledges
Dynamic person with strong value commitment
Good computer skills: solid knowledge of Microsoft Office and the ability to learn appropriate software.