Research: Blocking the Hedgehog-dependent tumor growth by a new selective Endoplasmic Reticulum Aminopeptidase 1 inhibitor
WORK ADDRESS: Sapienza University of Rome, Laboratory of Molecular Oncology, Department of Molecular Medicine, Viale Regina Elena 291, Roma.
SUPERVISORS: Lucia Di Marcotullio, Francesca Bufalieri
PREVIOUS RESEARCH EXPERIENCES: Laboratory of Clinical and Pathological Analysis - Hospital Agency “Papardo”, Messina (Italy).
CAREER:
- Bachelor’s degree in Biological sciences (L-13, D.M. 270/2010), Vote 107/110.
- Master’s degree in Neurobiology (LM-6, D.M 2017), Vote 110/110 summa cum laude.
SKILLS:
Good communication skills, acquired during the studies in Italy
and good ability to work in team, in international groups and in
multidisciplinary projects.
Good organizational skills acquired during the studies in Italy
and during my Ph.D. activity: good autonomy, ability to plan
and organize projects. High predisposition to teamwork and
multidisciplinary project organization. High flexibility and
adaptability.
Data analysis: Adobe Photoshop, Image J, Image Lab,
GraphPad. Use of various DataBases and tools: NCBI, PDB,
BLAST, GenScript, OncoMX, Uniprot, Biorender, STRING.
Cell culture: Primary cells (murine medulloblastoma cells),
immortalized murine and human cell lines, patient derived organoids (medulloblastoma, glioblastoma)
Immunofluorescence and histology: Tissue preparation of
histological samples: fixation, freezing, immunofluorescence.
Cell preparation for immunofluorescence: fixation and
permeabilization.
Molecular Biology techniques: Plasmidic and genomic DNA
extraction, DNA mutagenesis, cloning, RNA extraction, reverse
transcription, PCR, quantitative real time PCR, ubiquitination
assays, gel electrophoresis, luciferase report assays.
Analysis of proteins: Protein extraction and western blot
analysis, protein post-translational modifications analysis; in
vivo and in vitro immunoprecipitation.
Cellular biology: DNA and siRNA transfection, cell treatments,
BrdU and EDU assay, production and use of lentiviral vectors.
Animal (scientific procedures): manipulation of mice and
mice’s brain tissues, tail cutting, toe clipping.