ANGELA MARCHETTI

Dottoressa di ricerca

ciclo: XXXIV


supervisore: Prof.ssa Marianna Villano

Titolo della tesi: Optimisation of biotechnological processes for polyhydroxyalkanoates production from food industry by-products

This thesis is part of the European Horizon 2020 project, entitled “USABLE Packaging” (Unlocking the potential of Sustainable BiodegredabLe Packaging, BBI JU-Project ID: 836884). The aim of this project was to develop plastic materials that are biodegradable and bio-based, and that does not compete with the food chain or cause land use problems, thus implementing a correct circular economy in the plastics sector. To date, the continuous impoverishment of the land, the increase in atmospheric emissions and, in general, the increase in pollution in all environmental sectors, has generated the interest of the scientific, social and political world in finding increasingly green and eco-sustainable solutions. Among the solutions proposed by the new circular economy model is undoubtedly the replacement of some non-recyclable and non-biodegradable materials with more eco-friendly alternatives. One of the most widely used materials in the world is plastic, a material that is as useful and versatile as it is harmful to the environment and human health if not disposal correctly at the end-of-life. Therefore, replacing fuel-based plastics with bio-based and biodegradable products would be beneficial on several fronts. Indeed, this would reduce the volume of waste dumped into the environment, the amount of greenhouse gases released into the atmosphere and all the environmental risks associated with these issues. Thus, biopolymers have emerged as a great alternative, as they come from renewable sources and can be biodegradable. Among the others, polyhydroxyalkanoates (PHAs) are one of the most promising bioplastics, as they are synthesised by microbial fermentation of renewable resources such as agro-industrial and urban waste, are biodegradable, biocompatible, non-toxic, with properties comparable to their petroleum-based counterparts. Thanks to its characteristics, PHA can be used for a wide range of applications, but above all it can overcome the problems caused especially by non-biodegradable packaging. In this context, the general objective of this PhD thesis was: The optimisation of the PHA production process by means of mixed microbial cultures and purple bacteria, testing different operating conditions to obtain a polymer while valorizing food industry by-products. To achieve this goal, specific objectives had to be realized: 1 Implementing sustainable production of volatile fatty acids as precursors in the production of PHA from MMC. 1.1 Evaluation of fermentation degree of food industry by-products through acidogenic fermentation process. Study performed through batch tests and semi-continuous stirred tank reactor. 2 Management of a sequencing batch reactor for the PHA-MMC production. 2.2 Study of the Influence of the Organic Loading Rate applied and of the cycle length using a synthetic acids mixture as substrate in the PHA-MMC production through a sequencing batch reactor in laboratory scale. 2.3 Influence of real feed, obtained from the acidogenic fermentation semi-continuous test, on selected biomass at different OLRs during the accumulation tests. 2.4 PHA-MMC production operated under different feeding conditions. 3 PHA production from photosynthetic micro culture and from phototrophic purple bacteria. 3.2 Study of permanent Feast regime, through a laboratory scale SBR, using real feed as a substrate in purple bacteria-PHA production.

Produzione scientifica

11573/1714853 - 2024 - Valorization of Reground Pasta By-Product through PHA Production with Phototrophic Purple Bacteria
Marchetti, A.; Palhas, M.; Villano, M.; Fradinho, J. - 01a Articolo in rivista
rivista: CATALYSTS (Basel: Molecular Diversity Preservation International) pp. 1-16 - issn: 2073-4344 - wos: WOS:001209936500001 (0) - scopus: 2-s2.0-85191306581 (0)

11573/1727752 - 2024 - 13C-Labelled Glucose Reveals Shifts in Fermentation Pathway During Cathodic Electro-Fermentation with Mixed Microbial Culture
Salvatori, G.; Giampaoli, O.; Marchetti, A.; Miccheli, A.; Virdis, B.; Sciubba, F.; Villano, M. - 01a Articolo in rivista
rivista: CHEMSUSCHEM (Weinheim : Wiley-VCH-Verl.) pp. - - issn: 1864-564X - wos: WOS:001354858900001 (0) - scopus: 2-s2.0-85208553298 (0)

11573/1695925 - 2023 - Efficient utilization of monosaccharides from agri-food byproducts supports Chlorella vulgaris biomass production under mixotrophic conditions
Angelini, Francesca; Bellini, Erika; Marchetti, Angela; Salvatori, Gaia; Villano, Marianna; Pontiggia, Daniela; Ferrari, Simone - 01a Articolo in rivista
rivista: ALGAL RESEARCH (Amsterdam : Elsevier) pp. - - issn: 2211-9264 - wos: WOS:001137297400001 (5) - scopus: 2-s2.0-85180463120 (6)

11573/1688607 - 2023 - Evaluation of the acidogenic fermentation potential of food industry by-products
Marchetti, A.; Salvatori, G.; Astolfi, M. L.; Fabiani, M.; Fradinho, J.; Reis, M. A. M.; Gianico, A.; Bolzonella, D.; Villano, M. - 01a Articolo in rivista
rivista: BIOCHEMICAL ENGINEERING JOURNAL (Elsevier BV:PO Box 211, 1000 AE Amsterdam Netherlands:011 31 20 4853757, 011 31 20 4853642, 011 31 20 4853641, EMAIL: nlinfo-f@elsevier.nl, INTERNET: http://www.elsevier.nl, Fax: 011 31 20 4853598) pp. 109029- - issn: 1369-703X - wos: WOS:001062171200001 (2) - scopus: 2-s2.0-85169901807 (2)

11573/1688732 - 2023 - Coupled Biological and Thermochemical Process for Plastic Waste Conversion into Biopolymers
Marzulli, F.; Musivand, S.; Arengi, M.; De Caprariis, B.; De Filippis, P.; Marchetti, A.; Majone, M.; Villano, M. - 01a Articolo in rivista
rivista: CHEMICAL ENGINEERING TRANSACTIONS (Milano : Sauro Pierucci Milano : AIDIC) pp. 469-474 - issn: 2283-9216 - wos: (0) - scopus: 2-s2.0-85165207272 (1)

11573/1669564 - 2023 - Biotic transformation products of sulfonamides in environmental water samples. High-resolution mass spectrometry-based tentative identification by a suspect screening approach
Montone, Carmela Maria; Giannelli Moneta, Benedetta; Aita, Sara Elsa; Capriotti, Anna Laura; Cerrato, Andrea; Laganà, Aldo; Marchetti, Angela; Piovesana, Susy; Villano, Marianna; Cavaliere, Chiara - 01a Articolo in rivista
rivista: JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS (Oxford ; New York : Pergamon Press Amsterdam : Elsevier Science) pp. 1-9 - issn: 0731-7085 - wos: WOS:000939536700001 (7) - scopus: 2-s2.0-85147961208 (7)

11573/1666113 - 2022 - Polyhydroxyalkanoates production by mixed microbial cultures in sequencing batch reactors operated under different feeding conditions
Marchetti, A.; Lorini, L.; Salvatori, G.; Gianico, A.; Majone, M.; Villano, M. - 01a Articolo in rivista
rivista: CHEMICAL ENGINEERING TRANSACTIONS (Milano : Sauro Pierucci Milano : AIDIC) pp. 163-168 - issn: 2283-9216 - wos: (0) - scopus: 2-s2.0-85134386086 (2)

11573/1637350 - 2022 - Electro-fermentation: sustainable bioproductions steered by electricity
Virdis, B.; D. Hoelzle, R.; Marchetti, A.; Boto, S. T.; Rosenbaum, M. A.; Blasco-Gomez, R.; Puig, S.; Freguia, S.; Villano, M. - 01a Articolo in rivista
rivista: BIOTECHNOLOGY ADVANCES (Elsevier Science Limited:Oxford Fulfillment Center, PO Box 800, Kidlington Oxford OX5 1DX United Kingdom:011 44 1865 843000, 011 44 1865 843699, EMAIL: asianfo@elsevier.com, tcb@elsevier.co.UK, INTERNET: http://www.elsevier.com, http://www.elsevier.com/locate/shpsa/, Fax: 011 44 1865 843010) pp. - - issn: 0734-9750 - wos: WOS:000794206100002 (31) - scopus: 2-s2.0-85129387661 (44)

11573/1568118 - 2019 - On the micromixing behavior of a spinning disk reactor for metallic Cu nanoparticles production
Marchetti, A.; Stoller, M. - 01a Articolo in rivista
rivista: APPLIED SCIENCES (Basel: MDPI AG, 2011-) pp. - - issn: 2076-3417 - wos: WOS:000484444100105 (4) - scopus: 2-s2.0-85070874580 (6)

11573/1304421 - 2019 - On the effect of specific boundary flux parameters on membrane process design
Stoller, M.; Marchetti, A.; Ochando Pulido, J. M. - 01a Articolo in rivista
rivista: CHEMICAL ENGINEERING TRANSACTIONS (Milano : Sauro Pierucci Milano : AIDIC) pp. 685-690 - issn: 2283-9216 - wos: (0) - scopus: 2-s2.0-85066975763 (0)

11573/1304429 - 2019 - Design of novel equipment capable to quickly produce efficient nanomaterials for use in environmental and sanitary emergencies
Stoller, Marco; Vuppala, Srikanth; Cheng, Khley; Traore, Mamadou; Marchetti, Angela; Kanaev, Andrei; Chiavola, Agostina - 01a Articolo in rivista
rivista: CHEMICAL ENGINEERING TRANSACTIONS (Milano : Sauro Pierucci Milano : AIDIC) pp. 187-192 - issn: 2283-9216 - wos: (0) - scopus: 2-s2.0-85067017911 (1)

11573/1304425 - 2019 - Continuous removal of Cr(VI) by lab-scale fixed-bed column packed with chitosan-nanomagnetite particles
Vuppala, S.; Marchetti, A.; Cianfrini, C.; Stoller, M. - 01a Articolo in rivista
rivista: CHEMICAL ENGINEERING TRANSACTIONS (Milano : Sauro Pierucci Milano : AIDIC) pp. 193-198 - issn: 2283-9216 - wos: (0) - scopus: 2-s2.0-85067019284 (1)

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