PERSONAL INFORMATION
Family name, First name: Andrea Ilari
ORCID: 0000-0002-7754-399X
Scopus Author ID: 6601938545
ResearcherID: T-5501-2017
Date of birth: 17/12/1968
Nationality: Italian
• EDUCATION
1998 PhD in Biophysics at the University of Roma “La Sapienza”. Dissertation:”Metal binding proteins: (a) "Sorcin (SOlubile Resistance related Calcium-bInding proteiN), a penta-EF calcium binding protein with a role in the calcium-mediated intracellular signal transduction"; (b) "Crystallization and preliminary X-ray diffraction studies on the ferritin from Listeria innocua"
1993 Master degree in Chemistry, summa cum laude, at the University of Roma “La Sapienza”. Thesis :” "Heme- protein interactions in the Horse spleen Myoglobin and in the homodimeric hemoglobin from Scapharca inaequivalvis. Fluorescence and optical absorption studies on the proteins reconstituted with Zn- e Protoporfirin IX";
• CURRENT POSITION
2007 – First researcher (senior scientist) at the Institute of Molecular Biology and Pathology of the Italian National Research Council (IBPM-CNR), Rome Italy
• PREVIOUS POSITIONS
2001-2007 Researcher at the IBPM-CNR
• FELLOWSHIPS AND AWARDS
1999-2001 Research fellowship: “Crystallization of macromolecules in microgravity”, at the “Istituto di Biologia e Patologia Molecolari”, Centro Nazionale delle Ricerche (Italian National Research Council) in collaboration with the ASI (Italian Space Agency).
1999 Research fellowship : “Structural dynamics of nitrite reductase”. In collaboration with the “Centre National de la Recherche scientifique”- Laboratoire de Cristallographie et Cristallisation des Macromolécules Biologiques – Marseille (France).
1998 Post-doctoral fellowship: “Research on resistance-related proteins of Mycobacteria”, Dep. of Genetics and Microbiology ,University of Pavia, Italy
• SUPERVISION OF GRADUATE STUDENTS AND POSTDOCTORAL FELLOWS
2007 – 2023 Supervisor of the following six Master Students:
1) Annarita Fiorillo, Francesco Di Chiaro, Alessandra Cipollone,
Master degree in Chemistry, Faculty of Mathematics, Physics and Natural Sciences Sapienza University, Rome Italy,
2) Stefano Mocci, Flavio Cennamo Master degree in Genetics and Molecular Biology
Faculty of Mathematics, Physics and Natural Sciences Sapienza University, Rome Italy
3) Sabrina Marsella Master degree Chemistry and Pharmaceutical Technologies, Faculty of Pharmacy and Medicine, Sapienza University, Rome Italy
2014-2023 Supervisor of four Ph.D student in Biochemistry: Lorenzo Antonelli, Aasia Bibi, Eris Bidollari, Gabriella Angiulli, Department of Biochemistry Science “A. Rossi Fanelli” Sapienza University, Rome, Italy
2021 – 2023 Supervisor of two postdoctoral fellows: Anastasia Liuzzi, Cécile Exertier, IBPM-CNR, Rome, Italy
• TEACHING ACTIVITIES
2021-2023 Lecturer of the course STRUCTURES AND INTERACTIONS BETWEEN BIOMOLECULES of the integrated course STRUCTURAL BIOLOGY FOR DRUG DESIGN of the COURSE OF STUDY IN ADVANCED BIOTECHNOLOGIES - LM-9, Faculty of Biosciences, University of Teramo
• ORGANISATION OF SCIENTIFIC MEETINGS
2023 - Fiesole 5-6 june 2023, scientific and organizing commettee member of the meeting “4nd AIC-BMM”
2022 Fiesole 23-24 may 2022, scientific and organizing commettee member of the meeting “3nd AIC-BMM”
2021, 7-9 june scientific and organizing commettee member of the meeting “on line” “2nd AIC-BMM meeting”
2019 Naples 4-7 september – Scientific commettee member of the Fifth Meeting of the Italian (AIC) and Spanish Crystallographic (GE3C) Associations
2019 10-11 october, Member of the scientific and organizing committee of the congress “Cryo-electron microscopy in structural biology: paving the way towards precision biomedicine and biotechnology”
2019 9 may Member of the scientific commettee of the congress IBPM-CNR “From model systems to therapies”.
2018 8 may Member of the scientific commettee “Health, disease and environmental research: biology, tools and applications”.
2018 Roma 25-28 June – Member of the scientific and organizing committee of the congress “3rd Joint AIC-SILS conference”. the third annual meeting of the Italian Crystallographic Association (AIC) and of the Italian Society for Synchrotron Light (SILS)
2014 Torino, 16-17 october, Member of the scientific committee of the congress Crystal (Cl)year
• INSTITUTIONAL RESPONSIBILITIES
2020 – Councilor of the Biological Macromolecules Section of the AIC (Italian Crystallography Association)
2015 – Member of the Commission appointed on 16 March 2015 with provision number 17, for the participation of the CNR in the IUCr (International Union of Crystallography).
2015 - Member of the scientific committee of LIRH (Lega Italiana Ricerca Huntington and related diseases)
2015 – 2020 Member of the Institute Council of the Institute of Molecular Biology and Pathology of the CNR.
2015- Principal Investigator of Biocrystal facility (https://biocrystalfacility.it/it/facility-di-biocristallografia/)
2014- Member of the committee of Ph.D In biochemistry, Dep. Of Biochemical Sciences, Sapienza University of Rome
• MEMBERSHIPS OF SCIENTIFIC SOCIETIES
2023 – Member, Research Network “COST Action CA21111 One Health drugs against parasitic vector borne diseases in Europe and beyond” (OneHealthdrugs)”
2010 – Member of the Italian Crystallographic Association (AIC)
2017 – Member of Italian Society of Biochemistry and Molecular Biology
• MAJOR COLLABORATIONS
• Prof. Ferdinando Squitieri, Advanced in vivo and in vitro technologies to study Juvenile Huntington Disease neuronal connectivity and its relationship with clinical and genetic factors Topic, Ospedale Casa Sollievo della Sofferenza
• Prof. Maria Laura Bolognesi, Proteolysis targeting chimeras (PROTACs) to treat Leishmaniasis – PROLEISH, Dep. Of Pharmacy and Biotechnology , University of Bologna
• Michele Saviano Ph. D., Potentiating the Italian Capacity for Structural Biology Services in Instruct-ERIC (ITACA.SB), Institute of Crystallography, Italian National Research Council
• Prof. Lucia Banci, Potentiating the Italian Capacity for Structural Biology Services in Instruct-ERIC (ITACA.SB), Dep. Of Chemistry, University of Florence, CERM (Italian Centre of Nuclear Magnetic Resonance) he Italian Instruct-ERIC Centre
• Prof. Andrew Kruse, Structure-function relationship in Sigma1 Receptor, Harvard Medical School, Blavatnik Institute of Biological Chemistry and Molecular Pharmacology.
• Prof. Sandra Gemma, Optimization of Potent and Specific Trypanothione Reductase Inhibitors: A Structure-Based Drug Discovery Approach, Dep. Of Biotech., Chem. and Pharm., University of Siena
• Ilaria Genovese, S1R expression localization and activation in Huntington Disease, Italian Institute of Technology (IIT).
Research activities
Research sectors
The mechanism of action of proteins was investigated by using protein crystallography and biochemical characterization. This included the structure-function relationship of enzymes and transporters as well as their role within signalling networks that involves protein-protein recognition and complex formation.
The knowledge of the molecular mechanism exploited by proteins to carry out their function and involved in pathological processes is the starting point to design novel, targeted therapeutic strategies. In this contest, the main endeavour has been the determination of protein structures by protein crystallography and, more recently by single particle cryo-electron microscopy. Protein biochemical and biophysical characterization was carried out using a wide array of methods (kinetics, protein engineering, spectroscopy). Moreover, Andrea Ilari’s research have been focused on structure-based drug design targeting proteins involved in different pathology. The main topics of Andrea Ilari’s research have been:
1) Structrure-function relationships in E. coli flavohemoglobin and in the following truncated hemoglobins: Bacillus subtilis truncated hemoglobin and Thermobifida fusca truncated hemoglobin.
2) The structure function relationships in Sorcin (SOluble Resistance related Calcium bInding proteiN) a protein involved in excitation-contraction-relaxation processes in the heart through the interaction with several Ca2+ channels and involved in MuntiDrug Resistance in cancer.
3) Structure function relationships in Dps (DNA binding proteins from starved cells) cage-like proteins involved in the bacterial-DNA protection from oxidative damage and used as bioreactor to produce metal-based nanoparticles.
4) Structure-function relationship in proteins involved in the trypanothione metabolism, a molecule which protect the Leishmania parasites (responsible for Leishmaniasis) from the ROS (Reactive Oxygen Species) produced by macrophages during the infection .
5) Structure-based drug design targeting Trypanothione Reductase a protein involved in trypanothione metabolism and essential for the Leshmania parasite survival in the human host. The design of new lead compound isperformed using modern crystallographic techniques as Fragment Based Screening.
6) Study of the proteins involved in Zinc transport in pathogenic bacteria. The study aims at designing a new antibiotic against Salmonella enterica which, as all the gram negative bacteria, develops very easily drug resistance.
7) Structure-function relationship in Sigma1 R (S1R) a neuroprotective protein. The study aims at finding new S1R agonist to be used to project new drugs to cure neurodegenerative diseases.
Recent Scientific Activities.
Invited Lectures (2014-2023)
2023 – 3th july 2023, Aula EXPO, AdRMI1 Centro Convegni Via Alfonso
Corti 12 (20133 Invited speaker al meeting ‘‘Target oriented Development of Novel Leishmanicidal Agents’’ with the lecture: ‘‘Trypanothione Reductase: a good target for finding new drugs against Leishmaniasis through structure based drug design"
2023 - Roma 30 agosto- 3 settembre. Comunicazione orale al Congresso SILS 2023 Annual Meeting of the Italian Synchrotron Radiation Society.
Titolo: A fragment-based approach targeting trypanothione reductase for the development of new antileishmanial agents.
2021. 6-8 september Parma, invited speaker to the XLIX congress of the Italian Crystallographic Association (AIC) with the keynote lecture “ Trypanothione Reductase, one target different approaches for the development of a broad spectrum trypanocidal drug”
2020. 26– 28 October. 2nd International Caparica Conference on Leishmaniasis 2020 Caparica – Portugal. Oral communication “Structure-based drug design on Trypanothione Reductase, a good drug target to find new lead compounds active against trypanosomatid chediseases”,
2020. 10 february Invited speaker to the congress INSTRUCT Italia with the oral presentation: “Structural Biology Italian Network: Instruct-ITALIA”
2018. 26-29 november, Rome, Italy, Invited speaker at the congress “World Structural and Molecular Conference,” with the key note lecture “Structure-Based Drug Design On Trypanothione Reductase To Find New Drugs Against Leishmaniasis”.
2018 Invited speaker at the “Fifth International Conference on Polyamines: Biochemical, Physiological and Clinical Perspectives, 2-7 september Ocean University e all’ Howard Civil Service International House,Taipei (Taiwan) with a keynote lecture:: “Enzymes of the trypanothione-based redox metabolism and anti-kinetoplastid drug discovery.”
2018. 8 may Invited speaker at the congress IBPM-CNR “Health, disease and environmental research: biology, tools and applications.” With a keynote lecture: “Protein crystallography at IBPM: function discovery of cage-like proteins and sorcin”.
2015. 6-7 december, Indian Institute of Technology, Guwahati “Recent developments in Medicinal Biotechnology and Structure-based drug designing”, Trypanothione reductase and Trypanothione peroxidase,key enzymes of Leishmania dithiol redox metabolism: structural, functional and inhibition studies on the enzymes of the trypanothione metabolism.”
2014. 31 ago-5 september, third International congress on polyamines, Itamambuca resort, Brasil “ Trypanothione, a spermidine derivative, necessary for Leishmania survival: structural and inhibition studies on the trypanothione metabolism enzymes”
Scientific congress organization (2014-2023)
2023 - - Centro Studi Ricerca e Formazione CISL –San Domenico, Firenze 5-6th june 2023, scientific and organizing commettee member of the meeting “4nd AIC-BMM”
2022 - Fiesole 23-24 may 2022, scientific and organizing commettee member of the meeting “3nd AIC-BMM”
2021, 7-9 june scientific and organizing commettee member of the meeting “on line” “2nd AIC-BMM meeting”
2019 -Naples 4-7 september – Scientific commettee member of theFifth Meeting of the Italian (AIC) and Spanish Crystallographic (GE3C) Associations
2019 -10-11 october, Andrea Ilari is a member of the scientific and organizing committee of the congress “Cryo-electron microscopy in structural biology: paving the way towards precision biomedicine and biotechnology”
2019- 9 may Andrea Ilari is a member of the scientific commettee of the congress IBPM-CNR “From model systems to therapies”.
2018- 8 may Andrea Ilari is a member of the scientific commettee “Health, disease and environmental research: biology, tools and applications”.
2018- Roma 25-28 June – Andrea Ilari is a member of the scientific and organizing committee of the congress “3rd Joint AIC-SILS conference”. the third annual meeting of the Italian Crystallographic Association (AIC) and of the Italian Society for Synchrotron Light (SILS)
2014- Torino, 16-17 october , Andrea Ilari is a member of the scientific committee of the congress Crystal (Cl)year
Research projects and research unit coordination ( 2014-2023)
2021-2024 IBPM ’RU leader at IBPM of workpackage 6 “Training, outreach, and networking” for he project “Potentiating the Italian Capacity for Structural Biology Services in Instruct-ERIC” Acronimo “ITACA.SB”, PNRR funds , Line of investment 3.1, “Fondo per la realizzazione di un sistema integrato di infrastrutture di ricerca e innovazione”,finanziato dall’Unione europea – NextGenerationEU CUP: B53C22001790006
2020-2024 Research Unit coordinator of the project FISR (Fondo Integrativo della Ricerca): “ Proteolysis targeting chimeras (PROTACs) to treat Leishmaniasis – PROLEISH”. Project number: FISR2019_03796 PROLEISH.
2020-2023 Coordinator of the project XCHEM, at Diamonds (Didcot, UK) “Fragment-based approach targeting the Trypanosomatid redox key enzyme Trypanothione reductase.”reference number LB25167
2020-2021 Coordinator of the project iNEXT ARIA, “Fragment-based approach targeting the Trypanosomatid redox key enzyme Trypanothione reductase.” PID: 13997
2018-2022 Research Unit coordinator of the project Ricerca Finalizzata 2016: RARE in rarity: “Advanced in vivo and in vitro technologies to study Juvenile Huntington Disease neuronal connectivity and its relationship with clinical and genetic factors”, reference number: RF 2016-02364123:
2014-2023 Research unit coordinator of the project COLLEZIONE DI COMPOSTI CHIMICI ED ATTIVITÀ DI SCREENING – Structural and functional studies on the trypanothione and polyamine metabolism enzymes” CUP: B56G15001140005
Patents
National patent: Pyrrolyl hydroxamates for use in the prevention and/or treatment of bacterial infections.
Concession date: 10-12-2014. Concession number: 0001412640
deposition date 06/06/2012, WIPO code: 10MI2012A000980,
inventors: Piera Valenti, Tiziana Natalizi Roberto Di Santo, Luca Pescatori Giuliana Cucuzzoli Crucitti, , Andrea Ilari, Emilia Chiancone
Bibliometric parameters
103 documents
4271 citations
H-index = 38 (SCOPUS)
Scientific papers
2023
1. Mattioli R, Ilari A, Colotti B, Mosca L, Fazi F, Colotti G. Doxorubicin and other anthracyclines in cancers: Activity, chemoresistance and its overcoming. Mol Aspects Med. 2023 Oct;93:101205. doi: 10.1016/j.mam.2023.101205.
2. Tito C, Genovese I, Giamogante F, Benedetti A, Miglietta S, Barazzuol L, Cristiano L, Iaiza A, Carolini S, De Angelis L, Masciarelli S, Nottola SA, Familiari G, Petrozza V, Lauriola M, Tamagnone L, Ilari A, Calì T, Valdivia HH, Valdivia CR, Colotti G, Fazi F. Sorcin promotes migration in cancer and regulates the EGF-dependent EGFR signaling pathways. Cell Mol Life Sci. 2023 Jul 13;80(8):202. doi: 10.1007/s00018-023-04850-4.
3. Pascarella G, Antonelli L, Narzi D, Battista T, Fiorillo A, Colotti G, Guidoni L, Morea V, Ilari A.Investigation of the Entry Pathway and Molecular Nature of σ1 Receptor Ligands International Journal of Molecular Sciences, 2023, 24(7), 6367
4. # Madia VN, Ialongo D, Patacchini E, Exertier C, Antonelli L, Colotti G, Messore A, Tudino V, Saccoliti F, Scipione L, Ilari A, Costi R, Di Santo R. Inhibition of Leishmania infantum Trypanothione Reductase by New Aminopropanone Derivatives Interacting with the NADPH Binding Site. Molecules. 1;28(1):338. doi: 10.3390/molecules28010338
5. Espinoza-Chávez, R. M., Salerno, A., Liuzzi, A., Ilari, A., Milelli, A., Uliassi, E., & Bolognesi, M. L. (2022). Targeted protein degradation for infectious diseases: From basic biology to drug discovery. ACS Bio and Med Chem Au doi:10.1021/acsbiomedchemau.2c00063
2022
6. # Battista T, Federico S, Brogi S, Pozzetti L, Khan T, Butini S, Ramunno A, Fiorentino E, Orsini S, Di Muccio T, Fiorillo A, Exertier C, Di Risola D, Colotti G, Gemma S, Ilari A, Campiani G. Optimization of Potent and Specific Trypanothione Reductase Inhibitors: A Structure-Based Drug Discovery Approach. ACS Infect Dis. 2022, 8(8):1687-1699. doi: 10.1021/acsinfecdis.2c00325..
7. Fiorillo A, Colotti G, Exertier C, Liuzzi A, Seghetti F, Salerno A, Caciolla J, Ilari A. Innovative Approach for a Classic Target: Fragment Screening on Trypanothione Reductase Reveals New Opportunities for Drug Design. Front Mol Biosci. 2022, 9:900882. doi: 10.3389/fmolb.2022.900882. PMID: 35860359;
2021
8. # Annarita Fiorillo; Veronica Morea; Gianni Colotti; Andrea Ilari Huntingtin Ubiquitination Mechanisms and Novel Possible Therapies to Decrease the Toxic Effects of Mutated Huntingtin J. Pers. Med. 2021, Volume 11, Issue 12, 1309
9. Fiorillo A, Battistoni A, Ammendola S, Secli V, Rinaldo S, Cutruzzolà F, Demitri N, Ilari A. Structure and metal-binding properties of PA4063, a novel player in periplasmic zinc trafficking by Pseudomonas aeruginosa. ActaCrystallogr D Struct Biol. 2021 Nov 1;77(Pt 11):1401-1410. doi:10.1107/S2059798321009608.
10. Barile A, Battista T, Fiorillo A, di Salvo ML, Malatesta F, Tramonti A, Ilari A, Contestabile R. Identification and characterization of the pyridoxal 5'-phosphate allosteric site in Escherichia coli pyridoxine 5'-phosphate oxidase. J Biol Chem. 2021 Jan-Jun;296:100795. doi: 10.1016/j.jbc.2021.100795.
11. Alexander LT, Lepore R, Kryshtafovych A, Adamopoulos A, Alahuhta M, Arvin AM, Bomble YJ, Böttcher B, Breyton C, Chiarini V, Chinnam NB, Chiu W, Fidelis K, Grinter R, Gupta GD, Hartmann MD, Hayes CS, Heidebrecht T, Ilari A, Joachimiak A, Kim Y, Linares R, Lovering AL, Lunin VV, Lupas AN, Makbul C, Michalska K, Moult J, Mukherjee PK, Nutt WS, Oliver SL, Perrakis A, Stols L, Tainer JA, Topf M, Tsutakawa SE, Valdivia-Delgado M, Schwede T. Target highlights in CASP14: Analysis of models by structure providers. Proteins. 2021 Dec;89(12):1647-1672. doi: 10.1002/prot.26247.
12. # Battista T, Pascarella G, Staid DS, Colotti G, Rosati J, Fiorillo A, Casamassa A, Vescovi AL, Giabbai B, Semrau MS, Fanelli S, Storici P, Squitieri F, Morea V, Ilari A. Known Drugs Identified by Structure-Based Virtual Screening Are Able to Bind Sigma-1 Receptor and Increase Growth of Huntington Disease Patient-Derived Cells. Int J Mol Sci. 2021 Jan 28;22(3):1293. doi:10.3390/ijms22031293. PMID: 33525510; PMCID: PMC7865886.
13. # Chiarini V, Fiorillo A, Camerini S, Crescenzi M, Nakamura S, Battista T,Guidoni L, Colotti G, Ilari A. Structural basis of ubiquitination mediated by protein splicing in early Eukarya. Biochim Biophys Acta Gen Subj. 2021May;1865(5):129844. doi: 10.1016/j.bbagen.2021.129844. Epub 2021 Jan 11. PMID:33444728.
14. # Mosca L, Ilari A, Fazi F, Assaraf YG, Colotti G. Taxanes in cancer treatment:Activity, chemoresistance and its overcoming. Drug Resist Updat. 2021Jan;54:100742. doi: 10.1016/j.drup.2020.100742. Epub 2021 Jan 9. PMID: 33429249.
2020
15. # Ilari, A., Bellapadrona, G., Carbonera, D. et al. Disclosing the Molecular Mechanism of Iron Incorporation in Listeria innocua Dps by EPR Spectroscopy. Appl Magn Reson 51, 1543–1557 (2020). doi: 10.1007/s00723-020-01287-x
16. #Genovese I, Giamogante F, Barazzuol L, Battista T, Fiorillo A, Vicario M, D'Alessandro G, Cipriani R, Limatola C, Rossi D, Sorrentino V, Poser E, Mosca L, Squitieri F, Perluigi M, Arena A, van Petegem F, Tito C, Fazi F, Giorgi C, Calì T, Ilari A, Colotti G. Sorcin is an early marker of neurodegeneration, Ca2+ dysregulation and endoplasmic reticulum stress associated to neurodegenerative diseases. Cell Death Dis. 2020 Oct 15;11(10):861. doi: 10.1038/s41419-020-03063-y. PMID: 33060591; PMCID: PMC7566454.
17. #Turcano, L; Battista, T; De Haro ; Missineo, A; Alli, C ; Paonessa, G; Colotti, G; Harper, S; Fiorillo, A; Ilari, A; Bresciani, A Spiro-containing derivatives show antiparasitic activity against Trypanosoma brucei through inhibition of the trypanothione reductase enzyme PLOS NEGLECTED TROPICAL DISEASES 2020, 14(5), e0008339
18. Battista T, Colotti G, Ilari A, Fiorillo A. Targeting Trypanothione Reductase, a Key Enzyme in the Redox Trypanosomatid Metabolism, to Develop New Drugs against Leishmaniasis and Trypanosomiases. Molecules. 2020 Apr 21;25(8). pii: E1924. doi:10.3390/molecules25081924
19. Genovese I, Carotti A, Ilari A, Fiorillo A, Battista T, Colotti G, Ivarsson Y. Profiling calcium-dependent interactions between Sorcin and intrinsically disordered regions of human proteome. Biochim Biophys Acta Gen Subj. 2020 Apr 17;1864(8):129618. doi: 10.1016/j.bbagen.2020.129618.
20. # Battista T, Fiorillo A, Chiarini V, Genovese I, Ilari A, Colotti G. Roles of Sorcin in Drug Resistance in Cancer: One Protein, Many Mechanisms, for a Novel Potential Anticancer Drug Target. Cancers (Basel). 2020 Apr 6;12(4). pii: E887. doi:10.3390/cancers12040887
21. # Colotti G, Saccoliti F, Gramiccia M, Di Muccio T, Prakash J, Yadav S, Dubey VK, Vistoli G, Battista T, Mocci S, Fiorillo A, Bibi A, Madia VN, Messore A, Costi R, Di Santo R, Ilari A. Structure-guided approach to identify a novel class of anti-leishmaniasis diaryl sulfide compounds targeting the trypanothione metabolism. Amino Acids. 2020 Feb;52(2):247-259. doi: 10.1007/s00726-019-02731-4.
2019
22. # Carbo M, Brandi V, Pascarella G, Staid DS, Colotti G, Polticelli F, Ilari A, Morea V. Bioinformatics analysis of Ras homologue enriched in the striatum, a potential target for Huntington's disease therapy. Int J Mol Med. 2019 Dec;44(6):2223-2233. doi: 10.3892/ijmm.2019.4373. Epub 2019 Oct 15. PubMed PMID: 31638189; PubMed Central PMCID: PMC6844632.
2018
23. #Turcano L, Torrente E, Missineo A, Andreini M, Gramiccia M, Di Muccio T, Genovese I, Fiorillo A, Harper S, Bresciani A, Colotti G, Ilari A. Identification and binding mode of a novel Leishmania Trypanothione reductase inhibitor from high throughput screening. PLoS Negl Trop Dis. 2018 Nov 26;12(11):e0006969
24. Calisti L, Trabuco MC, Boffi A, Testi C, Montemiglio LC, des Georges A, Benni I, Ilari A, Taciak B, Białasek M, Rygiel T, Król M, Baiocco P, Bonamore A. Engineered ferritin for lanthanide binding. PLoS One. 2018 Aug 13;13(8):e0201859. doi: 10.1371/journal.pone.0201859.
25. Fiorillo A, Petrosino M, Ilari A, Pasquo A, Cipollone A, Maggi M, Chiaraluce R, Consalvi V. The phosphoglycerate kinase 1 variants found in carcinoma cells display different catalytic activity and conformational stability compared to the native enzyme. PLoS ONE 13(7): e0199191. doi: 10.1371/journal.pone.0199191
26. # Ilari A, Genovese I, Fiorillo F, Battista T, De Ionna I, Fiorillo A, Colotti G. Toward a Drug Against All Kinetoplastids: From LeishBox to Specific and Potent Trypanothione Reductase Inhibitors. Mol Pharm. 2018 Aug 6;15(8):3069-3078. doi: 10.1021/acs.molpharmaceut.8b00185.
27. Ortalli M, Ilari A, Colotti G, De Ionna I, Battista T, Bisi A, Gobbi S, Rampa A, Di Martino RMC, Gentilomi GA, Varani S, Belluti F. Identification of chalcone-based antileishmanial agents targeting trypanothione reductase. Eur J Med Chem. 2018 May 25;152:527-541. doi: 10.1016/j.ejmech.2018.04.057.
28. Bidollari E, Rotundo G, Ferrari D, Candido O, Bernardini L, Consoli F, De Luca A, Santimone I, Lamorte G, Ilari A, Squitieri F, Vescovi AL, Rosati J. Generation of induced pluripotent stem cell line, CSSi004-A (2962), from a patient diagnosed with Huntington's disease at the presymptomatic stage. Stem Cell Res. 2018 Apr;28:145-148. doi: 10.1016/j.scr.2018.02.014.
29. Ardini M, Howes BD, Fiorillo A, Falvo E, Sottini S, Rovai D, Lantieri M, Ilari A, Gatteschi D, Spina G, Chiancone E, Stefanini S, Fittipaldi M. Study of manganese binding to the ferroxidase centre of human H-type ferritin. J Inorg Biochem. 2018 May;182:103-112. doi: 10.1016/j.jinorgbio.2018.02.003.
30. # Colotti G, Fiorillo A, Ilari A. Metal- and metalloid-containing drugs for the treatment of trypanosomatid diseases. Front Biosci (Landmark Ed). 2018 Jan 1;23:954-966. Review. PubMed PMID: 28930584.
2017
31. Genovese I, Ilari A, Assaraf YG, Fazi F, Colotti G. Not only P-glycoprotein: Amplification of the ABCB1-containing chromosome region 7q21 confers multidrug resistance upon cancer cells by coordinated overexpression of an assortment of resistance-related proteins. Drug Resist Updat. 2017 May;32:23-46. doi: 10.1016/j.drup.2017.10.003. Epub 2017 Oct 16. Review.
32. Genovese I, Fiorillo A, Ilari A, Masciarelli S, Fazi F, Colotti G. Binding of doxorubicin to Sorcin impairs cell death and increases drug resistance in cancer cells. Cell Death Dis. 2017 Jul 20;8(7):e2950. doi: 10.1038/cddis.2017.342. PubMed PMID: 28726784; PubMed Central PMCID: PMC5550883.
33. # Battistoni A, Ammendola S, Chiancone E, Ilari A. A novel antimicrobial approach based on the inhibition of zinc uptake in Salmonella enterica. Future Med Chem. 2017 Jun;9(9):899-910. doi: 10.4155/fmc-20 17-0042. Epub 2017 Jun 21.Review. PubMed PMID: 28636417.
34. # Brogi S, Fiorillo A, Chemi G, Butini S, Lalle M, Ilari A, Gemma S, Campiani G. Structural characterization of Giardia duodenalis thioredoxin reductase (gTrxR) and computational analysis of its interaction with NBDHEX. Eur J Med Chem. 2017 Jul 28;135:479-490. doi: 10.1016/j.ejmech.2017.04.057.
35. # Morea V, Bidollari E, Colotti G, Fiorillo A, Rosati J, De Filippis L, Squitieri F, Ilari A. Glucose transportation in the brain and its impairment in Huntington disease: one more shade of the energetic metabolism failure? Amino Acids. 2017 Jul;49(7):1147-1157. doi: 10.1007/s00726-017-2417-2.
36. # Saccoliti F, Angiulli G, Pupo G, Pescatori L, Madia VN, Messore A, Colotti G, Fiorillo A, Scipione L, Gramiccia M, Di Muccio T, Di Santo R, Costi R, Ilari A. Inhibition of Leishmania infantum trypanothione reductase by diaryl sulfide derivatives. J Enzyme Inhib Med Chem. 2017 Dec;32(1):304-310. doi:10.1080/14756366.2016.1250755
37. # Ilari A, Fiorillo A, Genovese I, Colotti G. Polyamine-trypanothione pathway: an update. Future Med Chem. 2017 Jan;9(1):61-77. doi: 10.4155/fmc-2016-0180. Epub 2016 Dec 13. Review.
2016
38. Ilari A, Pescatori L, Di Santo R, Battistoni A, Ammendola S, Falconi M, Berlutti F, Valenti P, Chiancone E. Salmonella enterica serovar Typhimurium growth is inhibited by the concomitant binding of Zn(II) and a pyrrolyl-hydroxamate to ZnuA, the soluble component of the ZnuABC transporter. Biochim Biophys Acta. 2016 Mar;1860(3):534-41. doi: 10.1016/j.bbagen.2015.12.006.
2015
39. # Ilari A, Fiorillo A, Poser E, Lalioti VS, Sundell GN, Ivarsson Y, Genovese I, Colotti G. Structural basis of Sorcin-mediated calcium-dependent signal transduction. Sci Rep. 2015 Nov 18;5:16828. doi: 10.1038/srep16828.
40. Cau Y, Fiorillo A, Mori M, Ilari A, Botta M, Lalle M. Molecular Dynamics Simulations and Structural Analysis of Giardia duodenalis 14-3-3 Protein-Protein Interactions. J Chem Inf Model. 2015 Dec 28;55(12):2611-22. doi: 10.1021/acs.jcim.5b00452. Epub 2015 Nov 19. PubMed PMID: 26551337.
41. # Angiulli G, Lantella A, Forte E, Angelucci F, Colotti G, Ilari A, Malatesta F. Leishmania infantum trypanothione reductase is a promiscuous enzyme carrying an NADPH:O2 oxidoreductase activity shared by glutathione reductase. Biochim Biophys Acta. 2015 Sep;1850(9):1891-7. doi: 10.1016/j.bbagen.2015.05.022.
42. # Brindisi M, Brogi S, Relitti N, Vallone A, Butini S, Gemma S, Novellino E, Colotti G, Angiulli G, Di Chiaro F, Fiorillo A, Ilari A, Campiani G. Structure-based discovery of the first non-covalent inhibitors of Leishmania major tryparedoxin peroxidase by high throughput docking. Sci Rep. 2015 May 7;5:9705. doi: 10.1038/srep09705.
43. # Ilari A, Fiorillo A, Baiocco P, Poser E, Angiulli G, Colotti G. Targeting polyamine metabolism for finding new drugs against leishmaniasis: a review. Mini Rev Med Chem. 2015;15(3):243-52. Review.
2014
44. # Angelucci F, Morea V, Angelaccio S, Saccoccia F, Contestabile R, Ilari A. The crystal structure of archaeal serine hydroxymethyltransferase reveals idiosyncratic features likely required to withstand high temperatures. PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS 2014 Dec;82(12):3437-49. doi: 10.1002/prot.24697.
45. Colotti G, Poser E, Fiorillo A, Genovese I, Chiarini V, Ilari A. Sorcin, a calcium binding protein involved in the multidrug resistance mechanisms in cancer cells. Molecules. 2014 Sep 5;19(9):13976-89. doi: 10.3390/molecules190913976.
46. Fiorillo A, di Marino D, Bertuccini L, Via A, Pozio E, Camerini S, Ilari A, Lalle M. The crystal structure of Giardia duodenalis 14-3-3 in the apo form: when protein post-translational modifications make the difference. PLoS One. 2014;9(3):e92902. doi: 10.1371/journal.pone.0092902. eCollection 2014.
47. Lalioti VS, Ilari A, O'Connell DJ, Poser E, Sandoval IV, Colotti G. Sorcin links calcium signaling to vesicle trafficking, regulates polo-like kinase 1 and is necessary for mitosis. PLoS One. 2014 Jan 10;9(1):e85438. doi:10.1371/journal.pone.0085438. eCollection 2014.
48. Ilari A, Alaleona F, Tria G, Petrarca P, Battistoni A, Zamparelli C, Verzili D, Falconi M, Chiancone E. The Salmonella enterica ZinT structure, zinc affinity and interaction with the high-affinity uptake protein ZnuA provide insight into the management of periplasmic zinc. Biochim Biophys Acta. 2014 Oct 12;1840(1):535-544. doi: 10.1016/j.bbagen.2013.10.010.
2013
49. # Colotti G, Baiocco P, Fiorillo A, Boffi A, Poser E, Chiaro FD, Ilari A. Structural insights into the enzymes of the trypanothione pathway: targets for antileishmaniasis drugs. Future Med Chem. 2013 Oct;5(15):1861-75. Doi: 10.4155/fmc.13.146.
50. Colotti G, Ilari A, Fiorillo A, Baiocco P, Cinellu MA, Maiore L, Scaletti F, Gabbiani C, Messori L. Metal-Based Compounds as Prospective Antileishmanial Agents: Inhibition of Trypanothione Reductase by Selected Gold Complexes. ChemMedChem. 2013 Aug 23. doi: 10.1002/cmdc.201300276. [Epub ahead of print] PubMed PMID: 24039168.
51. # Baiocco P, Poce G, Alfonso S, Cocozza M, Porretta GC, Colotti G, Biava M, Moraca F, Botta M, Yardley V, Fiorillo A, Lantella A, Malatesta F, Ilari A. Inhibition of Leishmania infantum Trypanothione Reductase by Azole-Based Compounds: a Comparative Analysis with Its Physiological Substrate by X-ray Crystallography. Chem. Med. Chem. 2013, 8:1175-83.
52. Ardini M, Fiorillo A, Fittipaldi M, Stefanini S, Gatteschi D, Ilari A, Chiancone E. Kineococcus radiotolerans Dps forms a heteronuclear Mn-Fe ferroxidase center that may explain the Mn-dependent protection against oxidative stress. Biochim Biophys Acta. 2013, 1830:3745-55.
53. Colotti G, Ilari A, Boffi A, Morea V. Metals and Metal derivatives in Medicine. Mini Rev Med Chem. 2013, 13: 211-221.
2012
54. # Fiorillo A, Colotti G, Boffi A, Baiocco P, Ilari A. The crystal structures of the tryparedoxin-tryparedoxin peroxidase couple unveil the structural determinants of leishmania detoxification pathway. PLoS Negl Trop Dis. 2012, 6(8):e1781.
55. # Ilari A, Baiocco P, Messori L, Fiorillo A, Boffi A, Gramiccia M, Di Muccio T, Colotti G. A gold-containing drug against parasitic polyamine metabolism: the X-ray structure of trypanothione reductase from Leishmania infantum in complex with auranofin reveals a dual mechanism of enzyme inhibition. Amino Acids. 2012, 42, 803-811.
2011
56. Ilari A, Alaleona F, Petrarca P, Battistoni A, Chiancone E. The X-ray structure of the zinc transporter ZnuA from Salmonella enterica discloses a unique triad of zinc-coordinating histidines. J Mol Biol. 2011 Jun 17;409(4):630-41.
57. # Colotti G, Ilari A. Polyamine metabolism in Leishmania: from arginine to trypanothione. Amino Acids. 2011 Feb;40(2):269-85.
58. # Paola Baiocco, Andrea Ilari, Pierpaolo Ceci, Stefania Orsini, Marina Gramiccia, Trentina Di Muccio Inhibitory Effect of Silver Nanoparticles on Trypanothione Reductase Activity and Leismania infantum proliferation. ACS Med. Chem. Lett., 2011, 2 (3), 230–233.
59. # Fiorillo A, Federico R, Polticelli F, Boffi A, Mazzei F, Di Fusco M, Ilari A, Tavladoraki P. The structure of maize polyamine oxidase K300M mutant in complex with the natural substrates provides a snapshot of the catalytic mechanism of polyamine oxidation. FEBS J. 2011 278(5):809-21.
2010
60. # Kasyutich O, Ilari A, Fiorillo A, Tatchev D, Hoell A, Ceci P. Silver ion incorporation and nanoparticle formation inside the cavity of Pyrococcus furiosus ferritin: structural and size-distribution analyses. J. Am. Chem. Soc., 2010, 132(10):3621-7.
61. Alaleona F, Franceschini S, Ceci P, Ilari A, Chiancone E. Thermosynechococcus elongatus DpsA binds Zn(II) at a unique three histidine-containing ferroxidase center and utilizes O2 as ironoxidant with very high efficiency, unlike the typical Dps proteins. FEBS J. 2010, 277(4):903-17.
2009
62. Vivoli M, Angelucci F, Ilari A, Morea V, Angelaccio S, di Salvo ML, Contestabile R. Role of a conserved active site cation-pi interaction in Escherichia coli serine hydroxymethyltransferase. Biochemistry. 2009;48(50):12034-46.
63. # Baiocco P, Colotti G, Franceschini S, Ilari A. “Molecular basis of antimony treatment in leishmaniasis.” J Med Chem., 2009; 52(8):2603-12.
64. Baiocco P, Franceschini S, Ilari A, Colotti G. “Trypanothione reductase from Leishmania infantum: cloning, expression, purification, crystallization and preliminary X-ray data analysis.” Protein Pept Lett. 2009;16(2):196-200.
65. # Ilari A, Fiorillo A, Angelaccio S, Florio R, Chiaraluce R, van der Oost J, Consalvi V. “Crystal structure of a family 16 endoglucanase from the hyperthermophile Pyrococcus furiosus--structural basis of substrate recognition.” FEBS J., 2009;276(4):1048-58.
66. Ilari A, Franceschini S, Bonamore A, Arenghi F, Botta B, Macone A, Pasquo A, Bellucci L, Boffi A. “Structural basis of enzymatic (S)-norcoclaurine biosynthesis.” J Biol Chem. 2009; 284(2):897-904.
2008
67. # Pasquo A, Bonamore A, Franceschini S, Macone A, Boffi A, Ilari A. “Cloning, expression, crystallization and preliminary X-ray data analysis of norcoclaurine synthase from Thalictrum flavum.” Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008; 64:281-3.
68. Ilari A, Boffi A. “Structural studies on flavohemoglobins.” Methods Enzymol. 2008;436:187-202.
69. Ilari A, Bonamore A, Franceschini S, Fiorillo A, Boffi A, Colotti G. “The X-ray structure of N-methyltryptophan oxidase reveals the structural determinants of substrate specificity.” Proteins-Structure function and Bioinformatics. 2008;71(4):2065-75.
70. Franceschini S, Ilari A, Verzili D, Zamparelli C, Antaramian A, Rueda A, Valdivia HH, Chiancone E, Colotti G. “Molecular basis for the impaired function of the natural F112L sorcin mutant: X-ray crystal structure, calcium affinity, and interaction with annexin VII and the ryanodine receptor. FASEB J. 2008;22(1):295-306.
2007
71. Bellapadrona G, Chiaraluce R, Consalvi V, Ilari A, Stefanini S, Chiancone E. “The mutations Lys 114 --> Gln and Asp 126 --> Asn disrupt an intersubunit salt bridge and convert Listeria innocua Dps into its natural mutant Listeria monocytogenes Dps. Effects on protein stability at Low pH.” Proteins-Structure function and Bioinformatics. 2007;66(4):975-83.
72. Ilari A, Kjelgaard P, von Wachenfeldt C, Catacchio B, Chiancone E, Boffi A. “Crystal structure and ligand binding properties of the truncated hemoglobin from Geobacillus stearothermophilus.” Arch Biochem Biophys. 2007;457(1):85-94.
2006
73. # Franceschini S, Ceci P, Alaleona F, Chiancone E, Ilari A. “Antioxidant Dps protein from the thermophilic cyanobacterium Thermosynechococcus elongatus.” FEBS J. 2006; 273(21):4913-28.
2005
74. Ilari A., Latella M.C., Ceci P., Ribacchi F., Su M., Giangiacomo L., Stefanini S.,Chasteen N.D., Chiancone E. “The unusual intersubunit ferroxidase center of Listeria innocua Dps is required for hydrogen peroxide detoxification but not for iron uptake. A study with site-specific mutants”. Biochemistry, 44, 5579-87, (2005).
75. * Giangiacomo L, Ilari A, Boffi A, Morea V, Chiancone E. “The truncated oxygen-avid hemoglobin from Bacillus subtilis. X-ray structure and ligand binding properties.” J . Biol Chem., 2005, 280, 9192-202, (2005).
76. * Bonamore A, Ilari A, Giangiacomo L, Bellelli A, Morea V, Boffi A. “A novel thermostable hemoglobin from the actinobacterium Thermobifida fusca.” FEBS J. 2005; 272(16):4189-201.
77. Ceci P, Ilari A, Falvo E, Giangiacomo L, Chiancone E. “Reassessment of protein stability, DNA binding, and protection of Mycobacterium smegmatis Dps.” J Biol Chem. 2005;280(41):34776-85.
2004
78. # Ilari A, Angelaccio S, Fiorillo A, Florio R, Consalvi V, Chiaraluce R. “Crystallization and preliminary X-ray crystallographic analysis of the laminarinase endo-beta-1,3-glucanase from Pyrococcus furiosus.” Acta Crystallogr D Biol Crystallogr. 2004;60:2394-5.
79. Chiancone E, Ceci P, Ilari A, Ribacchi F, Stefanini S. “Iron and proteins for iron storage and detoxification.” Biometals. 2004;17(3):197-202. Review.
80. D'Angelo P, Lucarelli D, della Longa S, Benfatto M, Hazemann JL, Feis A, Smulevich G, Ilari A, Bonamore A, Boffi A. “Unusual heme iron-lipid acyl chain coordination in Escherichia coli flavohemoglobin.” Biophys J. 2004;86(6):3882-92.
2003
81. * Ceci P., Ilari A., Falvo E., Chiancone E., “The Dps protein of Agrobacterium tumefaciens does not bind to DNA but protects it toward oxidative cleavage: X-ray crystal structure, iron binding, and hydroxyl-radical scavenging properties.”J. Biol. Chem., 278, 20319-26, (2003).
82. Mella M, Colotti G, Zamparelli C, Verzili D, Ilari A, Chiancone E. “Information transfer in the penta-EF-hand protein sorcin does not operate via the canonical structural/functional pairing. A study with site-specific mutants.” J Biol Chem. 2003; 278(27):24921-8.
83. Bonamore A, Gentili P, Ilari A, Schininà ME, Boffi A. “Escherichia coli flavohemoglobin is an efficient alkylhydroperoxide reductase.” J Biol Chem. 2003; 278(25):22272-7.
2002
84. Ilari A., Ceci P., Ferrari D., Rossi G.L., Chiancone E.,”Iron incorporation into Escherichia coli Dps gives rise to a ferritin-like microcrystalline core” J. Biol. Chem.,277, 37619-23, (2002).
85. Zhao G., Ceci P., Ilari A., Giangiacomo L., Laue T.M., Chiancone E., Chasteen N.D. “Iron and hydrogen peroxide detoxification properties of DNA-binding protein from starved cells. A ferritin-like DNA-binding protein of Escherichia coli.” J Biol Chem. 2002; 277(31):27689-96.
86. Ilari A., Bonamore A., Farina A., Johnson .K. A., Boffi A., “ The X-ray structure of E. Coli flavohemoglobin reveals an unexpected geometry of the distal heme pocket.”, J. Biol. Chem., 277, 23725-32, (2002).
87. Ilari A., Johnson K. A., Nastopoulos V., Verzili D., Zamparelli C., Colotti G.,Tsernoglou D. and Chiancone E., “The crystal structure of the sorcin calcium binding domain provides a model of Ca2+-dependent processes in the full-length protein”, J. Mol. Biol., 317, 447-458, (2002).
2001
88. Nastopoulos V, Ilari A, Colotti G, Zamparelli C, Verzili D, Chiancone E, Tsernoglou D. “Two different crystal forms of sorcin, a penta-EF-hand Ca2+-binding protein.” Acta Crystallogr D Biol Crystallogr. 2001;57(Pt 6):862-4.
89. Boffi A, Bozzi M, Sciandra F, Woellner C, Bigotti MG, Ilari A, Brancaccio A. “Plasticity of secondary structure in the N-terminal region of beta-dystroglycan.” Biochim Biophys Acta- Protein structure and molecular Enzymology 2001;1546(1):114-21.
2000
90. Ilari, A., Stefanini, S., Tsernoglou, D., and Chiancone E., “ The dodecameric ferritin from L. innocua contains a novel intersubunit iron-binding site”, Nature Struct. Biol., 7, 38-43, (2000).
91. Chiaraluce R, Consalvi V, Cavallo S, Ilari A, Stefanini S, Chiancone E. “The unusual dodecameric ferritin from Listeria innocua dissociates below pH 2.0.” Eur J Biochem. (FEBS J.) 2000;267(18):5733-41.
92. Yang X, Chiancone E, Stefanini S, Ilari A, Chasteen ND. “Iron oxidation and hydrolysis reactions of a novel ferritin from Listeria innocua.” Biochem J. 2000 ; 349:783-6.
93. Zamparelli C, Ilari A, Verzili D, Giangiacomo L, Colotti G, Pascarella S, Chiancone E. “Structure-function relationships in sorcin, a member of the penta EF-hand family. Interaction of sorcin fragments with the ryanodine receptor and an Escherichia coli model system.” Biochemistry. 2000;39(4):658-66.
1999
94. Ilari A, Savino C, Stefanini S, Chiancone E, Tsernoglou D. “Crystallization and preliminary X-ray crystallographic analysis of the unusual ferritin from Listeria innocua.” Acta Crystallogr D Biol Crystallogr. 1999;55: 552-3.
1997
95. Zamparelli C, Ilari A, Verzili D, Vecchini P, Chiancone E. “Calcium- and pH-linked oligomerization of sorcin causing translocation from cytosol to membranes.” FEBS Lett. 1997; 409(1):1-6.
96. Boffi A, Zamparelli C, Verzili D, Ilari A, Chiancone E. “Effect of the vinyl-globin interactions on the temperature-dependent broadening of the Soret spectra: a study with horse myoglobin and Scapharca dimeric hemoglobin reconstituted with unnatural 2,4-heme derivatives.” Arch Biochem Biophys. 1997;340(1):43-51.
1996
97. Boffi A, Ilari A, Spagnuolo C, Chiancone E. “Unusual affinity of cyanide for ferrous and ferric Scapharca inaequivalvis homodimeric hemoglobin.” Equilibria and kinetics of the reaction. Biochemistry. 1996;35(24):8068-74.
1995
98. Ilari A, Boffi A, Chiancone E. “Rigidity of the heme pocket in the cooperative Scapharca hemoglobin homodimer and relation to the direct communication between hemes.” Arch Biochem Biophys. 1995;316(1):378-84.
# corresponding author; * first author
+ senior autorship
Book chapters
2020 Linda Celeste Montemiglio Adele Di Matteo, Carmelinda Savino, Barbara Illi, Andrea Ilari. Sars-Cov-2: frontiere della ricerca; Multimediale editor: Zanichelli, L’infezione alla luce della biologia strutturale. (https://sibbm.zanichelli.it/italiano/2020/08/23/infezione-biologia-strutturale/)
2019 Andrea Ilari, Gianni Colotti. Chapter 11. New Chemical Scaffolds to Selectively Target the Trypanothione Metabolism. Medicinal Chemistry of Neglected and Tropical Diseases, edited By Venkatesan Jayaprakash, Daniele Castagnolo, Yusuf Özkay
ISBN: 9781351011655
2018 –Colotti G., Ilari A. Sorcin. Encyclopedia of signaling Molecules, Second edition, Sangdun Choi edition, Pages 5084-5093
ISBN: 978-3-319-67198-7
2017- Ilari A, Savino C. A Practical Approach to Protein Crystallography. Methods Mol Biol. 1525:47-78.
PRINT ISBN: 978-1-4939-6620-2
ON LINE ISBN: 978-1-4939-6622-6
2015 –A. Ilari, A. Bonamore, and A. Boffi « Addition to C=N Bonds”, Book title: “Biocatalysis in Organic Synthesis 2”, Science of Synthesis (SOS) 2014-2016, Editor Thieme Chemistry.
ISBN: 9783131741714
2012- Pierpaolo Ceci, Veronica Morea, Manuela Fornara, Giuliano Bellapadrona, Elisabetta Falvo and Andrea Ilari “Biomimetic materials synthesis by ferritin-related, cage-shaped proteins”, Book title “Advanced Topics in Biomineralization”, 2012, Editor Intech - Open Access Publisher.
ISBN: 978-953-51-0045-4
2009 –Stefanini S., Ceci P., Ardini M., Ilari A.: DNA-binding proteins from sterved cells, Handbook of metalloproteins, edited by Prof. Messerschmidt, John Wiley & Sons, Ltd.
ISBN-10 : 0470849843
ISBN-13 : 978-0470849842
2008- Ilari A, Savino C. “Protein structure determination by X-ray crystallography.” Book title: “Bioinformatics: Data, Sequence Analysis and Evolution”, Editor Jonathan M. Keith, Series: Methods in Molecular Biology, Volume 452, pages 63-87.
ISBN: 1588297071