ALESSIA IAIZA

Dottoressa di ricerca

ciclo: XXXV


supervisore: Francesco Fazi

Titolo della tesi: Role of m6A-dependent circRNAs during stress response in myeloid leukemic cells

N6-methyladenosine (m6A) is one of the most prevalent, abundant and conserved RNA modifications. It controls several biological processes, including also the biogenesis and function of circular RNAs (circRNAs), which are a class of covalently closed single-stranded RNAs. Recently, it has emerged a molecular interaction between the m6A RNA modification factors and the suppression of the proteotoxic stress response which induction is emerging as a relevant anticancer therapy in Acute Myeloid Leukemia (AML). Since proteasome inhibition, leading to imbalance in protein homeostasis, is strictly linked to the stress response induction, we decided to investigate the effect of Bortezomib (Btz) on m6A enzymes expression and its impact on the modulation of m6A-dependent circRNAs expression. In this thesis, we show that treatment of AML cells with Btz induces m6A enzymes downregulation at translation level. Moreover, we evidenced that this modulation is mediated by ROS generation and activation of the oxidative stress response. Indeed, administration of the reducing agent N-acetylcysteine inhibits Btz-mediated m6A enzymes downregulation. Moreover, despite the general decrease of m6A modification levels, we found that some circRNAs are more methylated and expressed upon Btz treatment compared to control samples. All together these data allow us to identify and investigate specific m6A-dependent circRNAs involved in the response to proteotoxic stress generated by Btz, in order to exploit them as molecular targets to improve Btz effectiveness in AML.

Produzione scientifica

11573/1696800 - 2024 - Enhancing sensitivity of triple-negative breast cancer to DNA-damaging therapy through chemical inhibition of the m6A methyltransferase METTL3
Cesaro, Bianca; Iaiza, Alessia; Piscopo, Fabio; Tarullo, Marco; Cesari, Eleonora; Rotili, Dante; Mai, Antonello; Diana, Alberto; Londero, Michela; Del Giacco, Luca; Masetti, Riccardo; Di Leone, Alba; Naro, Chiara; Masciarelli, Silvia; Fontemaggi, Giulia; Sette, Claudio; Fazi, Francesco; Fatica, Alessandro - 01f Lettera, Nota
rivista: CANCER COMMUNICATIONS (London: BioMed Central) pp. 282-286 - issn: 2523-3548 - wos: WOS:001125441200001 (0) - scopus: 2-s2.0-85179653539 (0)

11573/1686664 - 2023 - Retinoic acid and proteotoxic stress induce AML cell death overcoming stromal cell protection
Liccardo, Francesca; Śniegocka, Martyna; Tito, Claudia; Iaiza, Alessia; Ottone, Tiziana; Divona, Mariadomenica; Travaglini, Serena; Mattei, Maurizio; Cicconi, Rosella; Miglietta, Selenia; Familiari, Giuseppe; Nottola, Stefania Annarita; Petrozza, Vincenzo; Tamagnone, Luca; Teresa Voso, Maria; Masciarelli, Silvia; Fazi, Francesco - 01a Articolo in rivista
rivista: JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH (London : BioMed Central) pp. 1-15 - issn: 1756-9966 - wos: WOS:001058414700001 (1) - scopus: 2-s2.0-85169408665 (1)

11573/1685494 - 2023 - Sorcin promotes migration in cancer and regulates the EGF-dependent EGFR signaling pathways
Tito, C.; Genovese, I.; Giamogante, F.; Benedetti, A.; Miglietta, S.; Barazzuol, L.; Cristiano, L.; Iaiza, A.; Carolini, S.; De Angelis, L.; Masciarelli, S.; Nottola, S. A.; Familiari, G.; Petrozza, V.; Lauriola, M.; Tamagnone, L.; Ilari, A.; Cali, T.; Valdivia, H. H.; Valdivia, C. R.; Colotti, G.; Fazi, F. - 01a Articolo in rivista
rivista: CELLULAR AND MOLECULAR LIFE SCIENCES (Basel: Birkhäuser 1997-) pp. 1-20 - issn: 1420-9071 - wos: WOS:001030311100002 (1) - scopus: 2-s2.0-85164843158 (1)

11573/1634413 - 2022 - Long Non-Coding RNAs in the Cell Fate Determination of Neoplastic Thymic Epithelial Cells
Iaiza, A.; Tito, C.; Ganci, F.; Sacconi, A.; Gallo, E.; Masciarelli, S.; Fontemaggi, G.; Fatica, A.; Melis, E.; Petrozza, V.; Venuta, F.; Marino, M.; Blandino, G.; Fazi, F. - 01g Articolo di rassegna (Review)
rivista: FRONTIERS IN IMMUNOLOGY (Lausanne : Frontiers Research Foundation, 2010-) pp. 1-10 - issn: 1664-3224 - wos: WOS:000876158300001 (0) - scopus: 2-s2.0-85129597994 (0)

11573/1610957 - 2022 - METTL3 contribution to cell proliferation in Thymic Epithelial Tumor Cells
Iaiza, Alessia; Tito, Claudia; Ianniello, Zaira; Ganci, Federica; Laquintana, Valentina; Gallo, Enzo; Sacconi, Andrea; Masciarelli, Silvia; De Angelis, Luciana; Aversa, Sara; Diso, Daniele; Anile, Marco; Petrozza, Vincenzo; Facciolo, Francesco; Melis, Enrico; Pescarmona, Edoardo; Venuta, Federico; Marino, Mirella; Blandino, Giovanni; Fontemaggi, Giulia; Fatica, Alessandro; Fazi, Francesco - 04f Poster
congresso: EMBO Workshop: The epitranscriptome (Heidelberg; Germany)
libro: Poster a congresso - ()

11573/1651452 - 2022 - Circular RNAs activity in the leukemic bone marrow microenvironment
Liccardo, F.; Iaiza, A.; Sniegocka, M.; Masciarelli, S.; Fazi, F. - 01g Articolo di rassegna (Review)
rivista: NON-CODING RNA (Basel : MDPI) pp. 1-14 - issn: 2311-553X - wos: WOS:000845800400001 (2) - scopus: 2-s2.0-85133651335 (3)

11573/1650849 - 2022 - MALAT1-dependent hsa_circ_0076611 regulates translation rate in triple-negative breast cancer
Turco, Chiara; Esposito, Gabriella; Iaiza, Alessia; Goeman, Frauke; Benedetti, Anna; Gallo, Enzo; Daralioti, Theodora; Perracchio, Letizia; Sacconi, Andrea; Pasanisi, Patrizia; Muti, Paola; Pulito, Claudio; Strano, Sabrina; Ianniello, Zaira; Fatica, Alessandro; Forcato, Mattia; Fazi, Francesco; Blandino, Giovanni; Fontemaggi, Giulia - 01a Articolo in rivista
rivista: COMMUNICATIONS BIOLOGY ([London]: Springer Nature) pp. 1-17 - issn: 2399-3642 - wos: WOS:000812308700006 (6) - scopus: 2-s2.0-85132079710 (9)

11573/1571404 - 2021 - METTL3-dependent MALAT1 delocalization drives c-Myc induction in thymic epithelial tumors
Iaiza, A.; Tito, C.; Ianniello, Z.; Ganci, F.; Laquintana, V.; Gallo, E.; Sacconi, A.; Masciarelli, S.; De Angelis, L.; Aversa, S.; Diso, D.; Anile, M.; Petrozza, V.; Facciolo, F.; Melis, E.; Pescarmona, E.; Venuta, F.; Marino, M.; Blandino, G.; Fontemaggi, G.; Fatica, A.; Fazi, F. - 01a Articolo in rivista
rivista: CLINICAL EPIGENETICS (Berlin : Springer) pp. 173- - issn: 1868-7083 - wos: WOS:000696541400003 (20) - scopus: 2-s2.0-85115179423 (19)

11573/1575650 - 2021 - New insight into the catalytic -dependent and -independent roles of METTL3 in sustaining aberrant translation in chronic myeloid leukemia
Ianniello, Z.; Sorci, M.; Ceci Ginistrelli, L.; Iaiza, A.; Marchioni, M.; Tito, C.; Capuano, E.; Masciarelli, S.; Ottone, T.; Attrotto, C.; Rizzo, M.; Franceschini, L.; De Pretis, S.; Voso, M. T.; Pelizzola, M.; Fazi, F.; Fatica, A. - 01a Articolo in rivista
rivista: CELL DEATH & DISEASE (Nature Group) pp. 1-12 - issn: 2041-4889 - wos: WOS:000698956000001 (18) - scopus: 2-s2.0-85115718682 (20)

11573/1610222 - 2021 - New insight into the catalytic -dependent and -independent roles of METTL3 in sustaining aberrant translation in Chronic Myeloid Leukemia
Ianniello, Zaira; Sorci, Melissa; Ceci Ginistrelli, Lavinia; Iaiza, Alessia; Marcella, Marchioni; Tito, Claudia; Capuano, Ernestina; Masciarelli, Silvia; Tiziana, Ottone; Cristina, Attrotto; Manuela, Rizzo; Luca, Franceschini; Stefano De Pretis, ; Maria Teresa Voso, ; Mattia, Pelizzola; Fazi, Francesco; Fatica, Alessandro - 04f Poster
congresso: 23rd Annual John Goldman E-Conference o​n Chronic Myeloid Leukemia: Biology and Therapy (Online)
libro: European School of Haematology - ()

11573/1567117 - 2021 - Circulating microRNAs from the molecular mechanisms to clinical biomarkers. A focus on the clear cell renal cell carcinoma
Tito, Claudia; De Falco, Elena; Rosa, Paolo; Iaiza, Alessia; Fazi, Francesco; Petrozza, Vincenzo; Calogero, Antonella - 01g Articolo di rassegna (Review)
rivista: GENES (Basel : Molecular Diversity Preservation International) pp. - - issn: 2073-4425 - wos: WOS:000689270500001 (12) - scopus: 2-s2.0-85111722975 (11)

11573/1452251 - 2020 - Liquid biopsy in clear cell renal cell carcinoma. Urinary miR-210-3p as emerging specific biomarker
Costantini, M.; Petrozza, V.; Tito, C.; Giammusso, L. M.; Sorrentino, V.; Cacciotti, J.; Porta, N.; Iaiza, A.; Pastore, A. L.; Di Carlo, A.; Simone, G.; Gallucci, M.; Carbone, A.; Fazi, F. - 04d Abstract in atti di convegno
rivista: EUROPEAN UROLOGY OPEN SCIENCE (Amsterdam: Elsevier B.V.) pp. - - issn: 2666-1691 - wos: (0) - scopus: (0)
congresso: EAU Virtual Congress 2020 (Online)
libro: EUROPEAN UROLOGY OPEN SCIENCE - ()

11573/1396163 - 2020 - LIQUID BIOPSY IN CLEAR CELL RENAL CELL CARCINOMA: URINARY MIR-210-3P AS EMERGING SPECIFIC BIOMARKER
Costantini, M; Petrozza, V; Tito, C; Giammusso, Lm; Sorrentino, V; Cacciotti, J; Porta, N; Iaiza, A; Pastore, Al; Di Carlo, A; Simone, G; Gallucci, M; Carbone, A; Fazi, F - 04d Abstract in atti di convegno
rivista: THE JOURNAL OF UROLOGY (Lippincott, Williams & Wilkins:530 Walnut Street:Philadelphia, PA 19106:(800)638-3030, (301)223-2300, EMAIL: orders@lww.com, INTERNET: http://www.lww.com, Fax: (301)223-2320, (301)223-2320) pp. E107-E107 - issn: 0022-5347 - wos: WOS:000527010300343 (0) - scopus: (0)
congresso: Annual Meeting of the American-Urological-Association (AUA) (Washington, DC)
libro: Annual Meeting of the American-Urological-Association (AUA) - Programme & Abstracts Book - ()

11573/1340650 - 2020 - Emerging role of secreted miR-210-3p as potential biomarker for clear cell renal cell carcinoma metastasis
Petrozza, Vincenzo; Costantini, Manuela; Tito, Claudia; Giammusso, Laura Maria; Sorrentino, Veronica; Cacciotti, Jessica; Porta, Natale; Iaiza, Alessia; Pastore, Antonio Luigi; Di Carlo, Angelina; Simone, Giuseppe; Carbone, Antonio; Gallucci, Michele; Fazi, Francesco - 01a Articolo in rivista
rivista: DISEASE MARKERS. SECTION A, CANCER BIOMARKERS (Amsterdam ; Washington, DC ; Tokyo : IOS Press) pp. 181-188 - issn: 1574-0153 - wos: WOS:000516568200006 (17) - scopus: 2-s2.0-85080853130 (20)

11573/1457118 - 2020 - LINC00174 is a novel prognostic factor in thymic epithelial tumors involved in cell migration and lipid metabolism
Tito, C.; Ganci, F.; Sacconi, A.; Masciarelli, S.; Fontemaggi, G.; Pulito, C.; Gallo, E.; Laquintana, V.; Iaiza, A.; De Angelis, L.; Benedetti, A.; Cacciotti, J.; Miglietta, S.; Bellenghi, M.; Carè, A.; Fatica, A.; Diso, D.; Anile, M.; Petrozza, V.; Facciolo, F.; Alessandrini, G.; Pescarmona, E.; Venuta, F.; Marino, M.; Blandino, G.; Fazi, F. - 01a Articolo in rivista
rivista: CELL DEATH & DISEASE (Nature Group) pp. 1-13 - issn: 2041-4889 - wos: WOS:000591695300002 (26) - scopus: 2-s2.0-85095603160 (25)

11573/1389033 - 2019 - Long non-coding RNAs contribution to cell migration and lipid metabolism in thymic epithelial tumor cells
Tito, Claudia; Ganci, F.; Sacconi, A.; Gallo, E.; De Angelis, Luciana; Pulito, C.; Iaiza, A.; Cacciotti, J.; Masciarelli, S.; Facciolo, F.; Petrozza, V.; Pescarmona, E.; Venuta, F.; Marino, M.; Blandino, G.; Fazi, F. - 04d Abstract in atti di convegno
congresso: ABCD Congress 2019, Bologna 19-21 September (Bologna)
libro: ABCD Congress 2019 - Programme & Abstracts Book - ()

11573/1389597 - 2019 - Long non-coding RNAs contribution to cell migration and lipid metabolism in Thymic Epithelial Tumor Cells
Tito, Claudia; Ganci, Federica; Sacconi, Andrea; Gallo, Enzo; De Angelis, Luciana; Pulito, Claudio; Iaiza, Alessia; Cacciotti, Jessica; Masciarelli, Silvia; Facciolo, Francesco; Petrozza, Vincenzo; Pescarmona, Edoardo; Venuta, Federico; Marino, Mirella; Blandino, Giovanni; Fazi, Francesco - 04d Abstract in atti di convegno
congresso: 73° Congresso SIAI - Società Italiana di Anatomia e Istologia (Napoli - Centro Congressi Federico II)
libro: 73° Congresso SIAI - Società Italiana di Anatomia e Istologia - Programme & Abstracts Book - ()

© Università degli Studi di Roma "La Sapienza" - Piazzale Aldo Moro 5, 00185 Roma