Research: Investigation of Notch1 signaling pathway in response to G2 damage checkpoint activation in T-cell acute lymphoblastic leukemia
PERSONAL INFORMATIONS:
Name and surname: Alessandra Simeone
Date of birth: 02/05/1998
Nationality: Italian
Work address: Laboratory of Molecular Pathology (Department of Molecular Medicine), Viale Regina Elena 291, 00161 Rome, Italy
Telephone number: +39 3395802534
Email: alessandra.simeone@uniroma1.it
CAREER:
October 2022 - Master's Degree in Genetics and Molecular Biology at "La Sapienza" University, Rome, Italy
Thesis title: "Study of Notch signaling pathway in response to G2 damage checkpoint activation in T-cell acute lymphoblastic leukemia"
Supervisor: Prof. Claudio Talora - Department of Molecular Medicine, University "La Sapienza", Rome, Italy
October 2020 - Bachelor's Degree in Biological Sciences at the University of Campania "Luigi Vanvitelli", Caserta, Italy
Thesis title: "Causes and consequences of genomic imprinting defects in human reproduction"
Supervisor: Prof.ssa Flavia Cerrato - Department of Biological and Pharmaceutical Environmental Sciences and Technologies, University of Campania "Luigi Vanvitelli", Caserta, Italy
July 2017 - Classical High School Diploma at the "Giulio Douhet" Military Air Force School, Florence, Italy
OTHER CERTIFICATIONS:
ECDL Full Standard (Online Essentials, Computer Essentials, Spreadsheets, Word Processing, IT Security - Specialized Level, Presentation, Online Collaboration)
DELF B1
NATIVE LANGUAGE: Italian
OTHER LANGUAGES: English, French
PREVIOUS RESEARCH EXPERIENCE:
November 2022 - Present: PhD student in Molecular Medicine, Department of Molecular Medicine, University "La Sapienza", Rome, Italy
Tutor: Prof. Claudio Talora
April 2021 - October 2022: Experimental thesis
Department of Molecular Medicine, Viale Regina Elena 291, 00161, Rome.
Thesis title: "Study of Notch signaling pathway in response to G2 damage checkpoint activation in T-cell acute lymphoblastic leukemia".
External tutor: Prof. Claudio Talora
Internal tutor: Prof. Loretta Tuosto
SKILLS:
Molecular Biology Techniques: DNA and RNA extraction. Total and fractional cell protein extraction, purification, quantification and Western Blot analysis. Separation of nucleic acids on agarose gel, PCR (Polymerase Chain Reaction), RT-PCR (Retro Transcriptase-Polymerase Chain Reaction), Real-time RT-PCR. Preparation of solutions.
Cell Biology Techniques: cell culture techniques, proliferation assays, staining for cytofluorimetric analysis of surface and intracellular markers, cell cycle with PI and apoptosis assay, preparation of culture media, expansion of cell lines, freezing and thawing. Pharmacological treatments in vitro.
Equipment: Use of centrifuges, autoclave, spectrophotometer, sonicator, analytical balance, thermocycler, Ph-meter, Real-Time PCR System, Western Blotting equipment, chemical and biological hoods and cell biology equipment.
Theoretical and practical training course at the animal facility of the Dept. of Molecular Medicine.
IT SKILLS:
Knowledge of application software such as Microsoft and iOS; office suites; Adobe Photoshop; Image J; Pymol.
I use databases and tools: NCBI, PDB, BLAST, GenScript, Biorender, STRING.