Phd in MOLECULAR MEDICINE

Research: ACP2 role in HH pathway

Personal data:
Name and surname: Alberico Di Pinto
Date and place of birth: 11/09/1996, Terracina (LT), Italy
Nationality: Italian
Work Address: Laboratory Of Molecular Oncology (Department of Molecular Medicine)
Viale Regina Elena 291, 00161 Rome, Italy
Phone number: +39 3296976238
Mail address: alberico.dipinto@uniroma1.it

Education:
01/03/2022-30/10/2023
RESEARCH FELLOWSHIP AT THE CNR (NATIONAL RESEARCH COUNCIL)-IBPM INSTITUTE
January 2022- Master degree in GENOMIC, INDUSTRIAL AND ENVIRONMENTAL BIOTECHNOLOGY (LM-8)
Place: University of Rome La Sapienza
Final grade: 110 cum laude
Thesis:
The neddylation mechanism of Ubiquitin ligase "Cullin-RING" (CRLs) regulates the response to drought in Arabidopsis thaliana
Supervisor: Prof.ssa Giovanna Serino- Dept. of Molecular Genetics and Biology

March 2019- bachelor degree in AGRO-INDUSTRIAL BIOTECHNOLOGY (L-2)
Place: University of Rome La Sapienza
Thesis:
Characterization of the R197K mutant form of pyridoxine-5-phosphate oxidase of Escherichia coli
Supervisor: Prof. Martino Luigi Di Salvo- Dept. of Molecular Genetics and Biology


Research Experience:
01/03/2022-30/10/2023
Research fellowship at the CNR-IBPM (Institute of Molecular Biology and Pathology)
Supervisor: Giovanna Sessa

01/03/2023-30/10/2023
Partnership with CREA institute about the targeted Genome Editing by using CRISPR-CAS9
Supervisor: Fabio D'Orso

Technical skills and competence:
Isolation of DNA, RNA and proteins from cells, tissues, and bacteria;
PCR, RT-qPCR, Western Blot Analysis and Immunoprecipitation assay;
Primers design for cloning, for PCR and qRT-PCR;
CRISPR-CAS9 (Sa, Sp, Cpf1) targeted genome editing;
Gateway and Golden Gate cloning systems;
Use of research databases;
Use of the microscope and stereoscope;
Use of laboratory equipment;
Preparation of reagents and media.
Cellular Biology:
cell culture techniques (animal cells), DNA transfection/transduction, cell treatments (CHX e. g.).
Histology and Histopathology techniques: immunofluorescence, histochemical techniques.


IT Skills:
Office suite; Adobe photoshop; Image J; Image Lab; Use of databases per data research;
Soft use of RStudio.