Dottoressa di ricerca

ciclo: XXXV

relatore: Prof.ssa Daniela Uccelletti

Titolo della tesi: 2-hydroxyisobutyric acid (2-HIBA) modulates ageing and fat deposition in C.elegans animal model

2-hydroxyisobutyric acid (2-HIBA) is a metabolite found in human urine. Recent studies have shown that 2-HIBA levels increased in patients with obesity and hepatic steatosis, suggesting that it could potentially be involved in clinical conditions. 2-HIBA is associated with reduced bacterial diversity and the presence of F. prausnitzii in obese gut microbiota. We investigated how treatment with 2-HIBA affected the physiology of the model organism Caenorhabditis elegans, in both standard and High-Glucose Diet (HGD) growth conditions, by targeted transcriptomic, metabolomic analyses, Coherent Anti-Stokes Raman Scattering (CARS) and two-photon fluorescence microscopy. 2-HIBA in both conditions resulted particularly effective to extend the lifespan, delay ageing processes and stimulate the oxidative stress resistance in wild type nematodes through the activation of insulin/IGF-1 signaling (IIS), and p38 MAPK pathways. Lifespan extension was mediated by activation of SKN-1 transcriptional factor and moreover by peptide transporter PEP-2. 2-HIBA pro-longevity effect on C. elegans, appeared to be correlated to increase in tryptophan levels as a consequence of a reduced expression of 2,3-dioxygenase (TDO) in treated animals. On the other hand, 2-HIBA influenced lipid metabolism in independently manner; in No-GD worms 2-HIBA induced an increase in lipid droplets which instead decreased in HGD conditions suggesting the activation of different pathways. Therefore, this study represents a first step in understanding the impact of 2-HIBA on C.elegans animal model.

Produzione scientifica

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Farina, Antonella; Tartaglione, Sara; Preziosi, Adele; Mancini, Patrizia; Angeloni, Antonio; Anastasi, Emanuela - 01a Articolo in rivista
rivista: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (Basel: MDPI Center) pp. - - issn: 1422-0067 - wos: (0) - scopus: (0)

11573/1678003 - 2023 - ZnO Nanorods Create a Hypoxic State with Induction of HIF-1 and EPAS1, Autophagy, and Mitophagy in Cancer and Non-Cancer Cells
Aventaggiato, Michele; Preziosi, Adele; Cheraghi Bidsorkhi, Hossein; Schifano, Emily; Vespa, Simone; Mardente, Stefania; Zicari, Alessandra; Uccelletti, Daniela; Mancini, Patrizia; Lotti, Lavinia Vittoria; Sarto, Maria Sabrina; Tafani, Marco - 01a Articolo in rivista
rivista: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (Basel: MDPI Center) pp. - - issn: 1422-0067 - wos: WOS:000977678900001 (4) - scopus: 2-s2.0-85157966007 (4)

11573/1627870 - 2022 - Humoral Immune response to Comirnaty ({BNT}162b2) {SARS}-Cov2 {mRNA} vaccine in Thalassemia Major patients
Anastasi, Emanuela; Marziali, Marco; Preziosi, Adele; Berardelli, Elena; Annunziata Losardo, Anna; Ribersani, Michela; Pugliese, Pellegrina; Farina, Antonella; Mancini, Patrizia; Angeloni, Antonio - 01a Articolo in rivista
rivista: MICROBES AND INFECTION (Editions Scientifique & Medical Elsevier:23 Rue Linois, F 75724 Paris Cedex 15 France:011 33 1 71724646, INTERNET:, Fax: 011 33 1 71724664) pp. - - issn: 1286-4579 - wos: WOS:000868871700001 (6) - scopus: 2-s2.0-85134214592 (7)

11573/1664474 - 2022 - Milmed yeast alters the LPS-induced M1 microglia cells to Form M2 anti-inflammatory phenotype
Armeli, Federica; Mengoni, Beatrice; Maggi, Elisa; Mazzoni, Cristina; Preziosi, Adele; Mancini, Patrizia; Businaro, Rita; Lenz, Thomas; Archer, Trevor Cornelius Stuart - 01a Articolo in rivista
rivista: BIOMEDICINES (Basel : MDPI) pp. - - issn: 2227-9059 - wos: WOS:000900498300001 (3) - scopus: 2-s2.0-85144730353 (5)

11573/1634557 - 2022 - In Vitro and In vivo biocompatibility studies on engineered fabric with graphene nanoplatelets
Fanizza, Carla; Stefanelli, Mara; Risuglia, Anna; Bruni, Erika; Ietto, Federica; Incoronato, Federica; Marra, Fabrizio; Preziosi, Adele; Mancini, Patrizia; Sarto, Maria Sabrina; Uccelletti, Daniela - 01a Articolo in rivista
rivista: NANOMATERIALS (Basel : MDPI) pp. 1-19 - issn: 2079-4991 - wos: WOS:000795376300001 (5) - scopus: 2-s2.0-85128709732 (6)

11573/1661960 - 2022 - 2-hydroxyisobutyric acid (2-HIBA) modulates ageing and fat deposition in Caenorhabditis elegans
Schifano, Emily; Conta, Giorgia; Preziosi, Adele; Ferrante, Carino; Batignani, Giovanni; Mancini, Patrizia; Tomassini, Alberta; Sciubba, Fabio; Scopigno, Tullio; Uccelletti, Daniela; Miccheli, Alfredo - 01a Articolo in rivista
rivista: FRONTIERS IN MOLECULAR BIOSCIENCES (Lausanne : Frontiers Media S.A., 2014-) pp. 1-18 - issn: 2296-889X - wos: WOS:000897896300001 (4) - scopus: 2-s2.0-85144068548 (4)

11573/1550214 - 2021 - Biocompatibility and antibiofilm properties of calcium silicate-based cements: an in vitro evaluation and report of two clinical cases
Bossù, Maurizio; Mancini, Patrizia; Bruni, Erika; Uccelletti, Daniela; Preziosi, Adele; Rulli, Marco; Relucenti, Michela; Donfrancesco, Orlando; Iaculli, Flavia; Di Giorgio, Gianni; Matassa, Roberto; Salucci, Alessandro; Polimeni, Antonella - 01a Articolo in rivista
rivista: BIOLOGY (Basel : MDPI) pp. - - issn: 2079-7737 - wos: WOS:000665378900001 (13) - scopus: 2-s2.0-85107542090 (14)

11573/1584705 - 2021 - Leuconostoc mesenteroides strains isolated from carrots show probiotic features
Schifano, Emily; Tomassini, Alberta; Preziosi, Adele; Montes, Jorge; Aureli, Walter; Mancini, Patrizia; Miccheli, Alfredo; Uccelletti, Daniela - 01a Articolo in rivista
rivista: MICROORGANISMS (Basel : MDPI) pp. - - issn: 2076-2607 - wos: WOS:000728061500001 (10) - scopus: 2-s2.0-85118382094 (11)

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