EDUCATION March 2004 Ph.D. in Biochemistry, “Sapienza” University of Rome. 2003 Abilitazione alla professione di Chimico. July 2000 Degree in Chemistry, “Sapienza” Università di Roma WORK EXPERIENCE Since 2023 Senior Researcher at the Institute of Molecular Biology and Pathology – CNR, Rome. December 2009 - December 2022 Research scientist at the Institute of Molecular Biology and Pathology – CNR, Rome. November 2008 – December 2009: Permanent position as a technician at the Department of Biochemical Sciences, “Sapienza”
University of Rome. April 2006 – November 2008: Post-doctoral research contract
at the Department of Biochemical Sciences, “Sapienza” University of Rome. December 2003 – March 2006: Post-doctoral research contract
at the Department of Plant Biology, “Sapienza” University of Rome.
AWARDS AND HONOURS 2009 Academia Europaea Burgen Scholars Award 2009 with the following motivation: selected given the high scientific value of your research. Since 2019 Italian Delegate appointed by the SIB for INPEC (International Nodes Of Protein Engineering Centers, https://www.inpec.science/). ACADEMIC EXPERIENCE 2003 – 2019 Member, as an expert in the subject, of the "Chemistry and Propaedeutic Biochemistry" examination commissions for the degree courses in Medicine and Surgery and Medicine and Dentistry of the "Sapienza" University of Rome. 2014 – 2018 Integrative/Elective teaching activity for the Chemistry and Biochemical Propaedeutics Course of the Faculty of Medicine and Dentistry, channel C. “Sapienza” University of Rome Since 2015 Tutor activity Ph.D. Thesis in Biochemistry Since 2013 Tutor activity for Master Thesis Medical Biotechnology, Pharmaceutical Biotechnology, Chemistry. OTHER ACADEMIC ACTIVITIES 2023 Member of the evaluation commission for the awarding of a collaboration assignment c/o IBPM-CNR 2022 Member of the commission for the awarding of a research fellowship c/o IBPM – CNR 2021 Member of the judging final commission for the award of the title of PhD in Biomolecular and Pharmaceutical Sciences (XXXIII cycle) at the “G. D'Annunzio", Chieti 2020 President of the evaluation commission for the awarding of a research fellowship c/o IBPM – CNR 2019 Member of the evaluation commission for the awarding of a collaboration assignment c/o IBPM – CNR 2016 Invited seminar for the PhD school in Biochemistry, “Sapienza”, University of Rome 2008 Invited seminar for the PhD school in Biochemistry, “Sapienza”, University of Rome 2007:nvited seminar for the PhD school in Biotechnology, University of L’Aquila OTHER ACTIVITIES Since 2020 Member of NeuroNet, network CNR on Neurosciences. Since 2012 Co-founder and Member of the Biocrystal Facility (https://biocrystalfacility.it/it/facility-di-biocristallografia/) SCIENTIFIC SOCIETIES MEMBERSHIP Member of the Italian Society of Biochemistry (SIB). Member of the Italian Association of Crystallography (AIC) QUALIFICATIONS 2023 –2034 National Scientific Qualification Biochemistry (05/E1 - SSD BIO/10) - Full Professor 2015 –2024: National Scientific Qualification Molecular Biology (05/E2 - SSD BIO/11) - Associate Professor PARTICIPATION IN MEETING ORGANIZATION 2022 3rd Meeting of the Biological Macromolecules Section of the Italian Crystallography Association”, Fiesole (Fi), 23-24 May 2022. Chair person. 2022 “Workshop on Structural Bases of Drug Design”. L'Aquila, 23 September 2022. Member of the Scientific and Organizational Committee and Chair person. 2019 “Cryo-electron microscopy in structural biology: paving the way towards precision biomedicine and biotechnology. A conference in memory of Emilia Chiancone”. Rome, 10th-11th October 2019. Member of the organizing committee 2019. Fifth Meeting of the Italian (AIC) and Spanish Crystallographic (GE3C) Associations (MISCA V)". Napoli, 4-7 September 2019. Chair person 2018 “Third joint conference of the Italian Crystallographic Association (AIC) and of the Italian Synchrotron Radiation Society (SILS)”. Rome 25-28 June 2018. Member of the Scientific Committee and the Organizing Committee MAIN RESEARCH LINES The research interest focuses mainly on structural biology, integrated with studies of molecular biology, biochemistry and biophysics to clarify the relationships between protein structures and their functions. The main lines of research are. - plant development and defense mechanisms, and remodeling of the plant cell wall - Study of potential antibacterial targets, in particular against Pseudomonas aeruginosa - Studies of human proteins, such as nucleophosmin (NPM1) and the FMRP protein, both in physiological conditions and in pathological states. EDITORIAL AND REVIEWER ACTIVITY - “Associate Editor” of Theoretical Modeling, Structure Prediction & Design (specialty section of Frontiers in Chemical Biology). -“Guest Editor” for Frontiers in Molecular Biosciences for the research topic “Tailored Modulation of Interactions between Biomolecules: Fundamentals and Applications” - Refereeing activity for scientific publications on BMC Plant Biology, International Journal of Molecular Sciences, Plants, Cells, Scientific Reports, Redox Biology PROJECT COORDINATION Since 2022 Responsible for the Institute of Molecular Biology and Pathology of WP3 "Widening services for biophysical characterization of proteins and their interactions" of the PNRR “ITACA.SB” project. 2019 - 2023 Operational Unit coordination of the PRIN 2017. Project financed by the MUR entitled "Regulatory signals and redox systems in plant growth-defense trade off". 2019 Coordinator of the “ELETTRA” Synchrotron Time Machine Project n. 20190203 2019 Coordinator of the “ELETTRA” Synchrotron Time Machine Project n. 20185502 2018 Coordinator of the “ELETTRA” Synchrotron Time Machine Project n. 20175169 2018 Coordinator of the “ELETTRA” Synchrotron Time Machine Project n. 20170545 2017 Coordinator of the “ELETTRA” Synchrotron Time Machine Project n. 20165282 2014 Coordinator of the “ELETTRA” Synchrotron Time Machine Project n. 20140559 ORAL PRESENTATIONS AT NATIONAL AND INTERNATIONAL CONFERENCES Catalano F., Santorelli D, Troilo F., Angelucci F., Fata F., Astegno A., Favretto F., D’Abramo M., De Sciscio M.L., Del Giudice A., Federici L., Demitri N., Giardina G., Travaglini-Allocatelli C., Di Matteo A. “The KH domains of FMRP: exploring folding, aggregation properties, and pathological variants”. SIB congress 2023. Firenze, 7-9 September 2023. Di Matteo A. Exploring nucleophosmin interactions for the treatment of acute myeloid leukemia. INPEC-APEM Proteins Conference, Camberra, AU. 5th December 2019. Di Matteo A. Tackling ribosome maturation process as antibacterial strategy: structural and functional investigation of RsgA from P. aeruginosa. IBPM annual meeting 2017, May 3th 2017, Rome. Di Matteo A., Federici L., Bonivento D., Bellincampi D., De Lorenzo G., Tsernoglou D. and Cervone F. “Signalling and Molecular Recognition in Plant Innate immunity”. Convegno FISV-SIFV. Bertinoro (Italy), 25-26 February 2005 Di Matteo A., Raiola A., Camardella L., Giovane A., Bonivento D., De Lorenzo G., Cervone F., Bellincampi D. and Tsernoglou D. “La struttura cristallografica del complesso tra la pectina metil esterasi ed il suo inibitore proteico a 1.9 Å di risoluzione”. “XXXIV Congresso Nazionale di Cristallografia”. Roma, 26-29 September 2004. Di Matteo A., Raiola A., Camardella L., Giovane A., Bonivento D., De Lorenzo G., Cervone F., Bellincampi D. and Tsernoglou D. “The 3D-structure of the complex between pectin methylesterase and pectin methylesterase inhibitor”. 10th International Cell Wall Meeting, Sorrento, 29 Agosto- 3 September 2004. Di Matteo A., Federici L., Mattei B., Cervone F. and Tsernoglou D. “The crystal structure of PGIP2 from Phaseolus vulgaris: new insight into the mode of interaction of a plant LRR protein”. Fifteenth John Innes Symposium “Structural Challenges in the Post-Genomic Era”. Norwich (UK), 15-18 July 2003 PARTICIPANT IN MAIN PROJECTS - PRIMA project (Partnership for Research and Innovation in the Mediterranean Area) entitled: “Fungal and enzymatic degradation of antibiotics: safe reuse of livestock residues for agriculture (FUNZYbio)”. Since 01-06-2023 - Project PNRR-PE13 entitled “One Health Basic and Translational Research Actions addressing Unmet Needs on Emerging Infectious Diseases – INFACT”. From 01-11-2022 to today - PNRR-IR project entitled “Potentiating the Italian Capacity for Structural Biology Services in Instruct-ERIC (ITACA.SB)”. Since 01-11-2022 to today - FISR_2019 project entitled “Proteolysis targeting chimeras (PROTACs) for the treatment of leishmaniasis (PROLEISH). Since 01-03-2021 to today PUBLICATIONS Scribani Rossi C, Eckartt K, Scarchilli E, Angeli S, Price-Whelan A, Di Matteo A, Chevreuil M, Raynal B, Arcovito A, Giacon N, Fiorentino F, Rotili D, Mai A, Espinosa-Urgel M, Cutruzzolà F, Dietrich LEP, Paone A, Paiardini A, Rinaldo S. Molecular insights into RmcA-mediated c-di-GMP consumption: Linking redox potential to biofilm morphogenesis in Pseudomonas aeruginosa. Microbiol Res. 2023 Dec;277:127498. doi: 10.1016/j.micres.2023.127498. Epub 2023 Sep 15. PMID: 37776579. Pedretti M, Favretto F, Troilo F, Giovannoni M, Conter C, Mattei B, Dominici P, Travaglini-Allocatelli C, Di Matteo A*, Astegno A. Role of myristoylation in modulating PCaP1 interaction with calmodulin. Plant Physiol Biochem. 2023 Oct;203:108003. doi: 10.1016/j.plaphy.2023.108003. Epub 2023 Sep 9. PMID: 7717348. Scafati V, Troilo F, Ponziani S, Giovannoni M, Scortica A, Pontiggia D, Angelucci F, Di Matteo A*, Mattei B, Benedetti M. Characterization of two 1,3-β-glucan-modifying enzymes from Penicillium sumatraense reveals new insights into 1,3-β-glucan metabolism of fungal saprotrophs. Biotechnol Biofuels Bioprod. 2022 Dec 12;15(1):138. doi: 10.1186/s13068-022-02233-8. PMID: 36510318; PMCID: PMC9745967. Santorelli D, Troilo F, Fata F, Angelucci F, Demitri N, Giardina G, Federici L, Catalano F, Di Matteo A*, Travaglini-Allocatelli C. Folding Mechanism and Aggregation Propensity of the KH0 Domain of FMRP and Its R138Q Pathological Variant. Int J Mol Sci. 2022 Oct 12;23(20):12178. doi: 10.3390/ijms232012178. PMID: 36293035; PMCID: PMC9603430. Ardini M, Baiocco P, Di Matteo A, Giardina G, Miele AE. Editorial: Tailored Modulation of Interactions Between Biomolecules: Fundamentals and Applications. Front Mol Biosci. 2022 Jul 14;9:961452. doi: 10.3389/fmolb.2022.961452. PMID: 35911973; PMCID: PMC9332331. Cela I, Cufaro MC, Fucito M, Pieragostino D, Lanuti P, Sallese M, Del Boccio P, Di Matteo A, Allocati N, De Laurenzi V, Federici L. Proteomic Investigation of the Role of Nucleostemin in Nucleophosmin-Mutated OCI-AML 3 Cell Line. Int J Mol Sci. 2022 Jul 11;23(14):7655. doi: 10.3390/ijms23147655. PMID: 35886999; PMCID: PMC9317519. Troilo F, Pedretti M, Travaglini-Allocatelli C, Astegno A, Di Matteo A.* (2022) Rapid kinetics of calcium dissociation from plant calmodulin and calmodulin-like proteins and effect of target peptides. Biochem Biophys Res Commun. 590:103-108. doi: 10.1016/j.bbrc.2021.12.077. Epub 2021 Dec 23. PMID:34974297. Conter C, Bombardi L, Pedretti M, Favretto F, Di Matteo A, Dominici P, Astegno A. (2021) The interplay of self-assembly and target binding in centrin 1 from Toxoplasma gondii. Biochem J. Jul 16;478(13):2571-2587. doi: 10.1042/BCJ20210295. PMID: 34114596; PMCID: PMC8286830. Fata F, Silvestri I, Ardini M, Ippoliti R, Di Leandro L, Demitri N, Polentarutti M, Di Matteo A, Lyu H, Thatcher GRJ, Petukhov PA, Williams DL, Angelucci F. (2021) Probing the Surface of a Parasite Drug Target Thioredoxin Glutathione Reductase Using Small Molecule Fragments. ACS Infect Dis. Jul 9;7(7):1932-1944. doi: 10.1021/acsinfecdis.0c00909. Epub 2021 May 5. PMID: 33950676; PMCID: PMC8273128. Santorelli D, Rocchio S, Fata F, Silvestri I, Angelucci F, Imperi F, Marasco D, Diaferia C, Gigli L, Demitri N, Federici L, Di Matteo A*, Travaglini-Allocatelli C. The folding and aggregation properties of a single KH-domain protein: Ribosome binding factor A (RbfA) from Pseudomonas aeruginosa. Biochim Biophys Acta Gen Subj. 2021 Feb;1865(2):129780. doi: 10.1016/j.bbagen.2020.129780. Epub 2020 Nov 4. PMID: 33157160. Mantoni F, Scribani Rossi C, Paiardini A, Di Matteo A, Cappellacci L, Petrelli R, Ricciutelli M, Paone A, Cutruzzolà F, Giardina G, Rinaldo S. Studying GGDEF Domain in the Act: Minimize Conformational Frustration to Prevent Artefacts. Life (Basel). 2021 Jan 6;11(1):31. doi: 10.3390/life11010031. PMID:n33418960; PMCID: PMC7825114. Di Natale C, Florio D, Di Somma S, Di Matteo A, Federici L, Netti PA, Morelli G, Malfitano AM, Marasco D. Proteostasis unbalance of nucleophosmin 1 in Acute Myeloid Leukemia: An aggregomic perspective. Int J Biol Macromol. 2020 Dec 1;164:3501-3507. doi: 10.1016/j.ijbiomac.2020.08.248. Epub 2020 Sep 2. PMID: 32890557. Cela I, Di Matteo A, Federici L. Nucleophosmin in Its Interaction with Ligands. Int J Mol Sci. 2020 Jul 10;21(14):4885. doi: 10.3390/ijms21144885. Scala R, Di Matteo A, Coluccia A, Lo Sciuto A, Federici L, Travaglini-Allocatelli C, Visca P, Silvestri R, Imperi F. Mutational analysis of the essential lipopolysaccharide-transport protein LptH of Pseudomonas aeruginosa to uncover critical oligomerization sites. Sci Rep. 2020 Jul 9;10(1):11276. doi:10.1038/s41598-020-68054-7. PMID: 32647254; PMCID: PMC7347655. Trande M, Pedretti M, Bonza MC, Di Matteo A, D'Onofrio M, Dominici P, Astegno A. Cation and peptide binding properties of CML7, a calmodulin-like protein from Arabidopsis thaliana. J Inorg Biochem. 2019 Oct;199:110796. doi: 10.1016/j.jinorgbio.2019.110796. Epub 2019 Aug 1. PMID: 31419675. Rocchio S, Santorelli D, Rinaldo S, Franceschini M, Malatesta F, Imperi F, Federici L, Travaglini-Allocatelli C, Di Matteo A*. Structural and functional investigation of the Small Ribosomal Subunit Biogenesis GTPase A (RsgA) from Pseudomonas aeruginosa. FEBS J. 2019 Nov;286(21):4245-4260. doi: 10.1111/febs.14959. Epub 2019 Jul 2. PMID: 31199072. Di Matteo A, Federici L, Masulli M, Carletti E, Santorelli D, Cassidy J, Paradisi F, Di Ilio C, Allocati N. Structural Characterization of the Xi Class Glutathione Transferase From the Haloalkaliphilic Archaeon Natrialba magadii. Front Microbiol. 2019 Jan 18;10:9. doi: 10.3389/fmicb.2019.00009. PMID: 30713525; PMCID: PMC6345682. Luchinat E, Chiarella S, Franceschini M, Di Matteo A, Brunori M, Banci L, Federici L. Identification of a novel nucleophosmin-interaction motif in the tumor suppressor p14arf. FEBS J. 2018 Mar;285(5):832-847. doi: 10.1111/febs.14373. Epub 2018 Jan 15. PMID: 29283500. De Cola A, Franceschini M, Di Matteo A, Colotti G, Celani R, Clemente E, Ippoliti R, Cimini AM, Dhez AC, Vallée B, Raineri F, Cascone I, Destouches D, De Laurenzi V, Courty J, Federici L. N6L pseudopeptide interferes with nucleophosmin protein-protein interactions and sensitizes leukemic cells to chemotherapy. Cancer Lett. 2018 Jan 1;412:272-282. doi: 10.1016/j.canlet.2017.10.038. Epub 2017 Oct 27. PMID: 29111347. De Santis A, La Manna S, Krauss IR, Malfitano AM, Novellino E, Federici L, De Cola A, Di Matteo A, D'Errico G, Marasco D. Nucleophosmin-1 regions associated with acute myeloid leukemia interact differently with lipid membranes. Biochim Biophys Acta Gen Subj. 2018 Apr;1862(4):967-978. doi: 10.1016/j.bbagen.2018.01.005. Epub 2018 Jan 10. PMID: 29330024. Di Matteo A, Franceschini M, Paiardini A, Grottesi A, Chiarella S, Rocchio S, Di Natale C, Marasco D, Vitagliano L, Travaglini-Allocatelli C, Federici L. Structural investigation of nucleophosmin interaction with the tumor suppressor Fbw7γ. Oncogenesis. 2017 Sep 18;6(9):e379. doi: 10.1038/oncsis.2017.78. PMID: 28920929; PMCID: PMC5623904. Di Matteo A, Franceschini M, Chiarella S, Rocchio S, Travaglini-Allocatelli C, Federici L. Molecules that target nucleophosmin for cancer treatment: an update. Oncotarget. 2016 Jul 12;7(28):44821-44840. doi: 10.18632/oncotarget.8599. PMID: 27058426; PMCID: PMC5190137. Di Natale C, Scognamiglio PL, Cascella R, Cecchi C, Russo A, Leone M, Penco A, Relini A, Federici L, Di Matteo A, Chiti F, Vitagliano L, Marasco D. Nucleophosmin contains amyloidogenic regions that are able to form toxic aggregates under physiological conditions. FASEB J. 2015 Sep;29(9):3689-701. doi: 10.1096/fj.14-269522. Epub 2015 May 14. PMID: 25977257. Arcovito A, Chiarella S, Della Longa S, Di Matteo A, Lo Sterzo C, Scaglione GL, Federici L. Synergic role of nucleophosmin three-helix bundle and a flanking unstructured tail in the interaction with G-quadruplex DNA. J Biol Chem. 2014 Aug 1;289(31):21230-41. doi: 10.1074/jbc.M114.565010. Epub 2014 Jun 21. PMID: 24952945; PMCID: PMC4118085. Benedetti M, Andreani F, Leggio C, Galantini L, Di Matteo A, Pavel NV, De Lorenzo G, Cervone F, Federici L, Sicilia F. A single amino-acid substitution allows endo-polygalacturonase of Fusarium verticillioides to acquire recognition by PGIP2 from Phaseolus vulgaris. PLoS One. 2013 Nov 19;8(11):e80610. doi: 10.1371/journal.pone.0080610. PMID: 24260434; PMCID: PMC3834070. Chiarella S, De Cola A, Scaglione GL, Carletti E, Graziano V, Barcaroli D, Lo Sterzo C, Di Matteo A, Di Ilio C, Falini B, Arcovito A, De Laurenzi V, Federici L. Nucleophosmin mutations alter its nucleolar localization by impairing G-quadruplex binding at ribosomal DNA. Nucleic Acids Res. 2013 Mar 1;41(5):3228-39. doi: 10.1093/nar/gkt001. Epub 2013 Jan 16. PMID: 23328624; PMCID: PMC359767 Di Silvio E, Di Matteo A, Malatesta F, Travaglini-Allocatelli C. Recognition and binding of apocytochrome c to P. aeruginosa CcmI, a component of cytochrome c maturation machinery. Biochim Biophys Acta. 2013 Aug;1834(8):1554-61. doi: 10.1016/j.bbapap.2013.04.027. Epub 2013 May 3. PMID: 23648553. Chiarella S, Federici L, Di Matteo A, Brunori M, Gianni S. The folding pathway of a functionally competent C-terminal domain of nucleophosmin: protein stability and denatured state residual structure. Biochem Biophys Res Commun. 2013 May 24;435(1):64-8. doi: 10.1016/j.bbrc.2013.04.038. Epub 2013 Apr 22. PMID: 23618861. Gallo A, Lo Sterzo C, Mori M, Di Matteo A, Bertini I, Banci L, Brunori M, Federici L. Structure of nucleophosmin DNA-binding domain and analysis of its complex with a G-quadruplex sequence from the c-MYC promoter. J Biol Chem. 2012 Aug 3;287(32):26539-48. doi: 10.1074/jbc.M112.371013. Epub 2012 Jun 15. PMID: 22707729; PMCID: PMC3410995. Federici L, Arcovito A, Scaglione GL, Scaloni F, Lo Sterzo C, Di Matteo A, Falini B, Giardina B, Brunori M. Nucleophosmin C-terminal leukemia-associated domain interacts with G-rich quadruplex forming DNA. J Biol Chem. 2010 Nov 26;285(48):37138-49. doi: 10.1074/jbc.M110.166736. Epub 2010 Sep 20. PMID: 20858903; PMCID: PMC2988320. Di Matteo A, Calosci N, Gianni S, Jemth P, Brunori M, Travaglini-Allocatelli C. Structural and functional characterization of CcmG from Pseudomonas aeruginosa, a key component of the bacterial cytochrome c maturation apparatus. Proteins. 2010 Aug 1;78(10):2213-21. doi: 10.1002/prot.22733. PMID: 20544959. Casasoli M, Federici L, Spinelli F, Di Matteo A, Vella N, Scaloni F, Fernandez-Recio J, Cervone F, De Lorenzo G. Integration of evolutionary and desolvation energy analysis identifies functional sites in a plant immunity protein. Proc Natl Acad Sci U S A. 2009 May 5;106(18):7666-71. doi: 10.1073/pnas.0812625106. Epub 2009 Apr 16. PMID: 19372373; PMCID: PMC2678593. Federici L, Lo Sterzo C, Pezzola S, Di Matteo A, Scaloni F, Federici G, Caccuri AM. Structural basis for the binding of the anticancer compound 6-(7-nitro-2,1,3-benzoxadiazol-4-ylthio)hexanol to human glutathione s-transferases. Cancer Res. 2009 Oct 15;69(20):8025-34. doi: 10.1158/0008-5472.CAN-09-1314. Epub 2009 Oct 6. PMID: 19808963. Bonivento D, Pontiggia D, Di Matteo A, Fernandez-Recio J, Salvi G, Tsernoglou D, Cervone F, De Lorenzo G, Federici L. Crystal structure of the endopolygalacturonase from the phytopathogenic fungus Colletotrichum lupini and its interaction with polygalacturonase-inhibiting proteins. Proteins. 2008 Jan 1;70(1):294-9. doi: 10.1002/prot.21610. PMID: 17876815. Giardina G, Rinaldo S, Johnson KA, Di Matteo A, Brunori M, Cutruzzolà F. NO sensing in Pseudomonas aeruginosa: structure of the transcriptional regulator DNR. J Mol Biol. 2008 May 16;378(5):1002-15. doi: 10.1016/j.jmb.2008.03.013. Epub 2008 Mar 14. PMID: 18420222. Di Matteo A, Scandurra FM, Testa F, Forte E, Sarti P, Brunori M, Giuffrè A. The O2-scavenging flavodiiron protein in the human parasite Giardia intestinalis. J Biol Chem. 2008 Feb 15;283(7):4061-8. doi: 10.1074/jbc.M705605200. Epub 2007 Dec 12. PMID: 18077462. Di Matteo A, Gianni S, Schininà ME, Giorgi A, Altieri F, Calosci N, Brunori M, Travaglini-Allocatelli C. A strategic protein in cytochrome c maturation: three-dimensional structure of CcmH and binding to apocytochrome c. J Biol Chem. 2007 Sep 14;282(37):27012-9. doi: 10.1074/jbc.M702702200. Epub 2007 Jul 10. PMID: 17623665. Federici L, Masulli M, Bonivento D, Di Matteo A, Gianni S, Favaloro B, Di Ilio C, Allocati N. Role of Ser11 in the stabilization of the structure of Ochrobactrum anthropi glutathione transferase. Biochem J. 2007 Apr 15;403(2):267-74. doi: 10.1042/BJ20061707. PMID: 17223798; PMCID: PMC1874244. Federici L, Di Matteo A, Fernandez-Recio J, Tsernoglou D, Cervone F. Polygalacturonase inhibiting proteins: players in plant innate immunity? Trends Plant Sci. 2006 Feb;11(2):65-70. doi: 10.1016/j.tplants.2005.12.005. Epub 2006 Jan 9. PMID: 16406303. Di Matteo A, Bonivento D, Tsernoglou D, Federici L, Cervone F. Polygalacturonase-inhibiting protein (PGIP) in plant defence: a structural view. Phytochemistry. 2006 Mar;67(6):528-33. doi: 10.1016/j.phytochem.2005.12.025. Epub 2006 Feb 3. PMID: 16458942 Spadoni S, Zabotina O, Di Matteo A, Mikkelsen JD, Cervone F, De Lorenzo G, Mattei B, Bellincampi D. Polygalacturonase-inhibiting protein interacts with pectin through a binding site formed by four clustered residues of arginine and lysine. Plant Physiol. 2006 Jun;141(2):557-64. doi: 10.1104/pp.106.076950. Epub 2006 Apr 28. PMID: 16648220; PMCID: PMC1475430 Borgia A, Bonivento D, Travaglini-Allocatelli C, Di Matteo A, Brunori M. Unveiling a hidden folding intermediate in c-type cytochromes by protein engineering. J Biol Chem. 2006 Apr 7;281(14):9331-6. doi: 10.1074/jbc.M512127200. Epub 2006 Feb 1. PMID: 16452476. Travaglini-Allocatelli C, Gianni S, Dubey VK, Borgia A, Di Matteo A, Bonivento D, Cutruzzolà F, Bren KL, Brunori M. An obligatory intermediate in the folding pathway of cytochrome c552 from Hydrogenobacter thermophilus. J Biol Chem. 2005 Jul 8;280(27):25729-34. doi: 10.1074/jbc.M502628200. Epub 2005 May 9. PMID: 15883159. Di Matteo A, Giovane A, Raiola A, Camardella L, Bonivento D, De Lorenzo G, Cervone F, Bellincampi D, Tsernoglou D. Structural basis for the interaction between pectin methylesterase and a specific inhibitor protein. Plant Cell. 2005 Mar;17(3):849-58. doi: 10.1105/tpc.104.028886. Epub 2005 Feb 18. PMID: 15722470; PMCID: PMC1069703. Cozzetto D, Di Matteo A, Tramontano A. Ten years of predictions ... and counting. FEBS J. 2005 Feb;272(4):881-2. doi: 10.1111/j.1742-4658.2005.04549.x. PMID: 15691322. Di Matteo A, Federici L, Mattei B, Salvi G, Johnson KA, Savino C, De Lorenzo G, Tsernoglou D, Cervone F. The crystal structure of polygalacturonase-inhibiting protein (PGIP), a leucine-rich repeat protein involved in plant defense. Proc Natl Acad Sci U S A. 2003. 100 (17): 0124-10128. doi: 10.1073/pnas.1733690100. PMID: 12904578 Federici L, Caprari C, Mattei B, Savino C, Di Matteo A, De Lorenzo G, Cervone F, Tsernoglou D. Structural requirements of endopolygalacturonase for the interaction with PGIP (polygalacturonase-inhibiting protein). Proc Natl Acad Sci U S A. 2001. 98 (23): 13425-13430. doi: 10.1073/pnas.231473698. PMID: 11687632